通过离子交换色谱法分离微管蛋白和微管相关蛋白。

Separation of tubulin and microtubule-associated proteins by ion exchange chromatography.

作者信息

Sloboda Roger D

机构信息

Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.

出版信息

Cold Spring Harb Protoc. 2015 Jan 5;2015(1):pdb.prot081208. doi: 10.1101/pdb.prot081208.

DOI:10.1101/pdb.prot081208

PMID:25561622

Abstract

Conventional liquid chromatography on phosphocellulose (PC) can be used to separate tubulin and microtubule-associated proteins (MAPs). Tubulin is a highly acidic protein and thus does not bind to PC. MAPs, however, do bind to PC and can be eluted with a subsequent salt wash of the column.

摘要

基于磷酸纤维素（PC）的传统液相色谱法可用于分离微管蛋白和微管相关蛋白（MAPs）。微管蛋白是一种高度酸性的蛋白质，因此不与PC结合。然而，MAPs确实能与PC结合，并可通过随后对柱子进行盐洗来洗脱。