Rozanov Alexey S, Bryanskaya Alla V, Malup Tatiana K, Meshcheryakova Irina A, Lazareva Elena V, Taran Oksana P, Ivanisenko Timofey V, Ivanisenko Vladimir A, Zhmodik Sergey M, Kolchanov Nikolay A, Peltek Sergey E
BMC Genomics. 2014;15 Suppl 12(Suppl 12):S12. doi: 10.1186/1471-2164-15-S12-S12. Epub 2014 Dec 19.
Geothermal areas are of great interest for the study of microbial communities. The results of such investigations can be used in a variety of fields (ecology, microbiology, medicine) to answer fundamental questions, as well as those with practical benefits. Uzon caldera is located in the Uzon-Geyser depression that is situated in the centre of the Karym-Semyachin region of the East Kamchatka graben-synclinorium. The microbial communities of Zavarzin spring are well studied; however, its benthic microbial mat has not been previously described.
Pyrosequencing of the V3 region of the 16S rRNA gene was used to study the benthic microbial community of the Zavarzin thermal spring (Uzon Caldera, Kamchatka). The community is dominated by bacteria (>95% of all sequences), including thermophilic, chemoorganotrophic Caldiserica (33.0%) and Dictyoglomi (24.8%). The benthic community and the previously examined planktonic community of Zavarzin spring have qualitatively similar, but quantitatively different, compositions.
In this study, we performed a metagenomic analysis of the benthic microbial mat of Zavarzin spring. We compared this benthic community to microbial communities found in the water and of an integral probe consisting of water and bottom sediments. Various phylogenetic groups of microorganisms, including potentially new ones, represent the full-fledged trophic system of Zavarzin. A thorough geochemical study of the spring was performed.
地热区对于微生物群落研究具有重要意义。此类调查结果可用于多个领域（生态学、微生物学、医学），以回答基础问题以及具有实际应用价值的问题。乌宗火山口位于乌宗-间歇泉洼地，该洼地位于堪察加半岛东部克拉夫-向斜褶皱带卡里姆-谢米亚钦地区的中心。扎瓦尔津泉的微生物群落已得到充分研究；然而，其底栖微生物垫此前尚未被描述。
利用16S rRNA基因V3区域的焦磷酸测序技术研究了扎瓦尔津温泉（堪察加半岛乌宗火山口）的底栖微生物群落。该群落以细菌为主（占所有序列的95%以上），包括嗜热的、化学有机营养型的钙热菌纲（33.0%）和网团菌纲（24.8%）。扎瓦尔津泉的底栖群落与之前检测的浮游群落组成在性质上相似，但在数量上有所不同。
在本研究中，我们对扎瓦尔津泉的底栖微生物垫进行了宏基因组分析。我们将这个底栖群落与泉水中以及由水和底部沉积物组成的整体样本中的微生物群落进行了比较。包括潜在新物种在内的各种微生物系统发育类群代表了扎瓦尔津完整的营养系统。我们还对该泉水进行了全面的地球化学研究。
