Caruso Rosario Alberto, Branca Giovanni, Fedele Francesco, Parisi Antonino, Finocchiaro Giuseppe, Ieni Antonio, Rigoli Luciana
Department of Human Pathology .
Ultrastruct Pathol. 2015;39(4):226-30. doi: 10.3109/01913123.2014.991886. Epub 2015 Jan 8.
A case of desmoplastic variant of diffuse-type gastric carcinoma in a 72-year-old woman is reported. Microscopic findings included poorly cohesive tumor cells, resembling mononuclear inflammatory cells, prominent diffuse desmoplasia, and tumor-associated tissue eosinophilia. Electron microscopy confirmed the undifferentiated phenotype of tumor cells and disclosed activated eosinophils in the tumor stroma. Eosinophil-specific granules were found either free in the tumor stroma or within the cytoplasm of some tumor cells. Electron microscopy provided also circumstantial evidence of phagocytosis of apoptotic eosinophils by tumor cells. Extracellular, membrane-bound, eosinophil-specific granules have been long recognized in tissues associated with eosinophilia, including allergic diseases, inflammatory responses to helminths, and in stroma of some neoplasms. Our ultrastructural study now extends these findings and provides additional morphological evidence of eosinophil-specific granules within the cytoplasm of gastric carcinoma cells.
报告了一例72岁女性弥漫型胃癌促纤维增生型变种病例。显微镜检查结果包括黏附性差的肿瘤细胞，类似单核炎性细胞，显著的弥漫性促纤维增生，以及肿瘤相关组织嗜酸性粒细胞增多。电子显微镜证实了肿瘤细胞的未分化表型，并揭示肿瘤基质中有活化的嗜酸性粒细胞。嗜酸性粒细胞特异性颗粒在肿瘤基质中游离存在，或存在于一些肿瘤细胞的细胞质内。电子显微镜还提供了肿瘤细胞吞噬凋亡嗜酸性粒细胞的间接证据。细胞外、膜结合的嗜酸性粒细胞特异性颗粒在与嗜酸性粒细胞增多相关的组织中早已被认识到，包括过敏性疾病、对蠕虫的炎症反应以及一些肿瘤的基质中。我们的超微结构研究现在扩展了这些发现，并提供了胃癌细胞质内嗜酸性粒细胞特异性颗粒的额外形态学证据。
