Bose Utpal, Hewavitharana Amitha K, Ng Yi Kai, Shaw Paul Nicholas, Fuerst John A, Hodson Mark P
School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia.
School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia.
Mar Drugs. 2015 Jan 7;13(1):249-66. doi: 10.3390/md13010249.
An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A "chemical profile" link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time.
基于液相色谱-质谱联用的代谢组学方法被用于表征由于生长培养基盐含量变化以及不同生长时期（培养时间）导致的次生代谢产物产量的变化。我们将代谢组学作为一种工具，研究从大堡礁（GBR）海绵中分离出的专性海洋放线菌盐孢菌在两种确定的盐浓度下以及三个不同培养时期的利福霉素（抗生素）和其他次生代谢产物的产生情况。结果表明，14天的培养期最有利于利福霉素B的最大产量，而利福霉素S和W在29天时达到最大浓度。培养天数与生长培养基盐浓度之间存在“化学图谱”联系，这可靠地代表了选择生长培养基和收获时间的关键点。
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