Kobayashi K, Tamaoki J, Sakai N, Kanemura T, Chiyotani A, Shibasaki T, Takizawa T
First Department of Medicine, Tokyo Women's Medical College, Japan.
Life Sci. 1989;45(21):2043-9. doi: 10.1016/0024-3205(89)90579-1.
To assess the effects of corticotropin-releasing factor (CRF) and adrenocorticotropin (ACTH) on airway ciliary activity, we measured ciliary beat frequency (CBF) by a photoelectric method in response to these peptides in cultured rabbit tracheal explants. When cumulatively added, both CRF and ACTH increased CBF in a dose-dependent fashion. Treatment of tissues with Ca2+-free medium or nifedipine abolished the effect of CRF but not of ACTH. The CRF- and ACTH-induced ciliostimulations were not affected by indomethacin or autonomic antagonists, but were attenuated by nordihydroguaiaretic acid and by their receptor antagonists, alpha-helical CRF (9-41) and ACTH (7-38). Intracellular cyclic AMP levels were significantly increased by CRF and ACTH. These results suggest that CRF and ACTH stimulate airway ciliary motility through the activation of adenylate cyclase and lipoxygenase by binding to their specific receptors, where the effect of CRF may be triggered by Ca2+ influx.
为评估促肾上腺皮质激素释放因子（CRF）和促肾上腺皮质激素（ACTH）对气道纤毛活动的影响，我们采用光电方法在培养的兔气管外植体中测量了纤毛摆动频率（CBF），以检测这些肽对其的反应。当累积添加时，CRF和ACTH均以剂量依赖方式增加CBF。用无钙培养基或硝苯地平处理组织可消除CRF的作用，但不影响ACTH的作用。CRF和ACTH诱导的纤毛刺激不受吲哚美辛或自主神经拮抗剂的影响，但可被去甲二氢愈创木酸及其受体拮抗剂α-螺旋CRF（9-41）和ACTH（7-38）减弱。CRF和ACTH可使细胞内环磷酸腺苷水平显著升高。这些结果表明，CRF和ACTH通过与其特异性受体结合激活腺苷酸环化酶和脂氧合酶，从而刺激气道纤毛运动，其中CRF的作用可能由Ca2+内流触发。
