Fernández-Zenoff María V, Estévez María C, Farías María E
Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (LIMLA), Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CCT, CONICET, Tucumán, Argentina; Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (LIMLA), Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CCT, CONICET, Tucumán, Argentina.
Rev Argent Microbiol. 2014 Oct-Dec;46(4):358-62. doi: 10.1016/S0325-7541(14)70095-1.
Laguna Azul is an oligotrophic lake situated at 4,560 m above sea level and subject to a high level of solar radiation. Bacterioplankton community composition (BCC) was analysed by denaturing gradient gel electrophoresis and the impact of solar ultraviolet radiation was assessed by measuring cyclobutane pyrimidine dimers (CPD). Furthermore, pure cultures of Acinetobacter johnsonii A2 and Rhodococcus sp. A5 were exposed simultaneously and CPD accumulation was studied. Gel analyses generated a total of 7 sequences belonging to Alpha-proteobacteria (1 band), Beta-proteobacteria (1 band), Bacteroidetes (2 bands), Actinobacteria (1 band), and Firmicutes (1 band). DGGE profiles showed minimal changes in BCC and no CPD was detected even though a high level of damage was found in biodosimeters. A. johnsonii A2 showed low level of DNA damage while Rhodococcus sp. A5 exhibited high resistance since no CPD were detected under natural UV-B exposure, suggesting that the bacterial community is well adapted to this highly solar irradiated environment.
拉古纳阿苏尔湖是一个贫营养湖，位于海拔4560米处，受到高强度的太阳辐射。通过变性梯度凝胶电泳分析了浮游细菌群落组成（BCC），并通过测量环丁烷嘧啶二聚体（CPD）评估了太阳紫外线辐射的影响。此外，同时对约翰逊不动杆菌A2和红球菌属A5的纯培养物进行了照射，并研究了CPD的积累情况。凝胶分析共产生了7个序列，分别属于α-变形菌纲（1条带）、β-变形菌纲（1条带）、拟杆菌门（2条带）、放线菌门（1条带）和厚壁菌门（1条带）。变性梯度凝胶电泳图谱显示BCC变化极小，尽管在生物剂量计中发现了高水平的损伤，但未检测到CPD。约翰逊不动杆菌A2显示出低水平的DNA损伤，而红球菌属A5表现出高抗性，因为在自然UV-B照射下未检测到CPD，这表明细菌群落很好地适应了这种高太阳辐射环境。
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