与HIV-1肽复合的树枝状大分子与脂质体和脂质单层相互作用。

Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers.

作者信息

Ionov Maksim, Ciepluch Karol, Garaiova Zuzana, Melikishvili Sophie, Michlewska Sylwia, Balcerzak Łucja, Glińska Sława, Miłowska Katarzyna, Gomez-Ramirez Rafael, de la Mata Francisco Javier, Shcharbin Dzmitry, Waczulikova Iveta, Bryszewska Maria, Hianik Tibor

机构信息

Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.

出版信息

Biochim Biophys Acta. 2015 Apr;1848(4):907-15. doi: 10.1016/j.bbamem.2014.12.025. Epub 2015 Jan 7.

DOI:10.1016/j.bbamem.2014.12.025

PMID:25576765

Abstract

AIMS

We have investigated the effect of surface charge of model lipid membranes on their interactions with dendriplexes formed by HIV-derived peptides and 2 types of positively charged carbosilane dendrimers (CBD).

METHODS

Interaction of dendriplexes with lipid membranes was measured by fluorescence anisotropy, dynamic light scattering and Langmuir-Blodgett techniques. The morphology of the complexes was examined by transmission electron microscopy.

RESULTS

All dendriplexes independent of the type of peptide interacted with model lipid membranes. Negatively charged vesicles composed of a mixture of DMPC/DPPG interacted more strongly, and it was accompanied by an increase in anisotropy of the fluorescent probe localized in polar domain of lipid bilayers. There was also an increase in surface pressure of the lipid monolayers. Mixing negatively charged liposomes with dendriplexes increased liposome size and made their surface charges more positive.

CONCLUSIONS

HIV-peptide/dendrimer complexes interact with model lipid membranes depending on their surface charge. Carbosilane dendrimers can be useful as non-viral carriers for delivering HIV-peptides into cells.

摘要