Christo Susan N, Diener Kerrilyn R, Nordon Robert E, Brown Michael P, Griesser Hans J, Vasilev Krasimir, Christo Farid C, Hayball John D
Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, SA 5000, Australia.
Sansom Institute and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
Sci Rep. 2015 Jan 14;5:7760. doi: 10.1038/srep07760.
The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.
在功能化表面捕获并激活单个T细胞，能够对细胞内钙释放的幅度和节律进行实时分析。应用哈尔小波变换可生成钙通量“阈值”，“阈值穿越”的频率与原始T细胞刺激的强度相关。这些发现代表了一种在单细胞水平实时评估T细胞激活程度的新方法。
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