Ramis Ivy Bastos, Cnockaert Margo, Von Groll Andrea, Mathys Vanessa, Simon Anne, Tortoli Enrico, Palomino Juan Carlos, Almeida da Silva Pedro Eduardo, Vandamme Peter, Andre Emmanuel, Martin Anandi
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium.
Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Pelotas, Brazil.
J Med Microbiol. 2015 Mar;64(Pt 3):283-287. doi: 10.1099/jmm.0.000025. Epub 2015 Jan 16.
Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. The present study aimed to evaluate the performance of the Speed-Oligo Mycobacteria assay for the identification of NTM. We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates) belonging to 13 different species (Mycobacterium intracellulare, M. fortuitum, M. gordonae, M. kansasii, M. marinum, M. peregrinum, M. scrofulaceum, M. abscessus, M. bovis BCG, M. chelonae, M. avium, M. malmoense and M. xenopi). The Speed-Oligo Mycobacteria assay was performed according to the manufacturer's instructions. Discrepant results between Speed-Oligo Mycobacteria and the original identification were reassessed by the Speed-Oligo Mycobacteria assay and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein-encoding genes. We found 93.5 % (58/62) concordance for the identification of NTM as compared with the original identification. Three strains were erroneously identified by Speed-Oligo Mycobacteria: one M. kansasii strain was identified as Mycobacterium tuberculosis complex, and one M. chelonae strain and one M. peregrinum strain were both identified as Mycobacterium abscessus. Moreover, one M. chelonae strain was not identified by Speed-Oligo Mycobacteria since it did not react with any species-specific probe. For these strains, sequencing of the genes hsp65, 16S rRNA and rpoB and the GenoType Mycobacterium CM assay were performed. The Speed-Oligo Mycobacteria assay can be a useful tool for the rapid and easy identification of the most common NTM. If applied in clinical practice it could reduce diagnostic delays and contribute to correct clinical and better management of infections caused by NTM.
引起人类感染性疾病的非结核分枝杆菌（NTM）已变得越来越常见。因此，快速准确地鉴定到种水平至关重要。Speed - Oligo分枝杆菌检测法是一种寡色谱法，最近可用于分枝杆菌的鉴定和区分。本研究旨在评估Speed - Oligo分枝杆菌检测法对NTM的鉴定性能。我们共检测了属于13个不同种（胞内分枝杆菌、偶然分枝杆菌、戈登分枝杆菌、堪萨斯分枝杆菌、海分枝杆菌、溃疡分枝杆菌、瘰疬分枝杆菌、脓肿分枝杆菌、卡介苗、龟分枝杆菌、鸟分枝杆菌、马尔默分枝杆菌和蟾分枝杆菌）的62株菌株（9株模式菌株、19株参考菌株和34株临床分离株）。按照制造商的说明进行Speed - Oligo分枝杆菌检测法。Speed - Oligo分枝杆菌检测法与原始鉴定结果之间的差异结果通过Speed - Oligo分枝杆菌检测法重新评估，并通过GenoType分枝杆菌CM检测法以及16S rRNA和蛋白质编码基因测序来解决。与原始鉴定相比，我们发现NTM鉴定的一致性为93.5%（58/62）。有3株菌株被Speed - Oligo分枝杆菌检测法错误鉴定：1株堪萨斯分枝杆菌菌株被鉴定为结核分枝杆菌复合群，1株龟分枝杆菌菌株和1株溃疡分枝杆菌菌株均被鉴定为脓肿分枝杆菌。此外，1株龟分枝杆菌菌株未被Speed - Oligo分枝杆菌检测法鉴定出来，因为它未与任何种特异性探针发生反应。对于这些菌株，进行了hsp65、16S rRNA和rpoB基因测序以及GenoType分枝杆菌CM检测法。Speed - Oligo分枝杆菌检测法可以成为快速简便鉴定最常见NTM的有用工具。如果应用于临床实践，它可以减少诊断延迟，并有助于对NTM引起的感染进行正确的临床诊断和更好的管理。
