通过抗体捕获VIDISCA-454技术鉴定一种新型人类鼻病毒C型
Identification of a novel human rhinovirus C type by antibody capture VIDISCA-454.
作者信息
Jazaeri Farsani Seyed Mohammad, Oude Munnink Bas B, Canuti Marta, Deijs Martin, Cotten Matthew, Jebbink Maarten F, Verhoeven Joost, Kellam Paul, Loens Katherine, Goossens Herman, Ieven Margareta, van der Hoek Lia
机构信息
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands.
Department of Virus Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK.
出版信息
Viruses. 2015 Jan 19;7(1):239-51. doi: 10.3390/v7010239.
Causative agents for more than 30 percent of respiratory infections remain unidentified, suggesting that unknown respiratory pathogens might be involved. In this study, antibody capture VIDISCA-454 (virus discovery cDNA-AFLP combined with Roche 454 high-throughput sequencing) resulted in the discovery of a novel type of rhinovirus C (RV-C). The virus has an RNA genome of at least 7054 nt and carries the characteristics of rhinovirus C species. The gene encoding viral protein 1, which is used for typing, has only 81% nucleotide sequence identity with the closest known RV-C type, and, therefore, the virus represents the first member of a novel type, named RV-C54.
超过30%的呼吸道感染病原体仍未明确，这表明可能存在未知的呼吸道病原体。在本研究中，抗体捕获VIDISCA-454（病毒发现cDNA-AFLP与罗氏454高通量测序相结合）发现了一种新型鼻病毒C（RV-C）。该病毒具有至少7054 nt的RNA基因组，并具有鼻病毒C种的特征。用于分型的病毒蛋白1编码基因与最接近的已知RV-C型仅有81%的核苷酸序列同一性，因此，该病毒代表了一种新型的首个成员，命名为RV-C54。
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