红棕灰夜蛾（Amsacta albistriga walker）对氯氰菊酯的抗性选育及抗性分子机制
Resistance selection and molecular mechanisms of cypermethrin resistance in red hairy caterpillar (Amsacta albistriga walker).
作者信息
Muthusamy R, Shivakumar M S
机构信息
Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India.
Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India.
出版信息
Pestic Biochem Physiol. 2015 Jan;117:54-61. doi: 10.1016/j.pestbp.2014.10.009. Epub 2014 Oct 22.
Amsacta albistriga is one of the important pests of oilseed crops in India. This pest has developed high resistance to organophosphate (OP) insecticide in field. Therefore, cypermethrin insecticide was used as an alternative for this pest. After 20 generations of selection with cypermethrin, the LD50 value for A. albistriga was increased by 21.5-folds. The synergism ratio of piperonyl butoxide (PBO) and triphenyl phosphate (TPP) was increased by 10- and 9.6-fold in resistant strains and comparatively, 3.9 and 4.2-fold in susceptible strains. Detoxification enzyme analysis and native PAGE electrophoresis of esterase isoenzyme further revealed that esterase and mixed function oxidase may be involved in cypermethrin resistance in CypRes strain. In addition to enzyme analysis overexpression of CYP4M44, CYP9A77 and CYP6B47 (ortholog) can confer metabolic resistance in the CypRes strain. These data provide a foundation for further study of cypermethrin resistance mechanism observed in A. albistriga.
白纹安螟是印度油料作物的重要害虫之一。这种害虫在田间已对有机磷（OP）杀虫剂产生了高抗性。因此，氯氰菊酯杀虫剂被用作防治这种害虫的替代品。在用氯氰菊酯进行20代选择后，白纹安螟的半数致死剂量（LD50）值增加了21.5倍。增效醚（PBO）和磷酸三苯酯（TPP）的增效比在抗性品系中分别增加了10倍和9.6倍，相比之下，在敏感品系中分别增加了3.9倍和4.2倍。解毒酶分析和酯酶同工酶的非变性聚丙烯酰胺凝胶电泳进一步表明，酯酶和混合功能氧化酶可能与氯氰菊酯抗性品系（CypRes品系）对氯氰菊酯的抗性有关。除了酶分析外，CYP4M44、CYP9A77和CYP6B47（直系同源基因）的过表达可使CypRes品系产生代谢抗性。这些数据为进一步研究在白纹安螟中观察到的氯氰菊酯抗性机制奠定了基础。
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