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没食子酸与环孢素联合应用对大鼠心肌形态缺血/再灌注损伤的保护作用。

Protective effects of co-administration of gallic Acid and cyclosporine on rat myocardial morphology against ischemia/reperfusion.

作者信息

Dianat Mahin, Sadeghi Najmeh, Badavi Mohammad, Panahi Marziyeh, Taheri Moghadam Mahin

机构信息

Physiology Research Center, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

Department of Histology and Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

出版信息

Jundishapur J Nat Pharm Prod. 2014 Oct 11;9(4):e17186. doi: 10.17795/jjnpp-17186. eCollection 2014 Nov.

Abstract

BACKGROUND

Irreversible myocardial ischemic injury begins 20 minutes after the onset of coronary occlusion. Then the infarcted cells show signs of necrosis and death.

OBJECTIVES

This study investigated the effects of co-administration of Gallic acid (antioxidant) with cyclosporine (mitochondrial permeability transition pore [mPTP] inhibitor) on myocardial morphology of rats during ischemia and reperfusion.

MATERIALS AND METHODS

Fifty-four male Wistar rats (250-300 g), were randomly divided into 9 groups: sham, control (Ca received saline, 1 mL/kg, Cb: perfused with cyclosporine CsA 0.2 µM), 3 groups pretreated with Gallic acid in saline (G1a:7.5, G2a:15, and G3a: 30 mg/kg/day, and gavage daily for 10 days, n = 6), and the other three groups were pretreated with Gallic acid then perfused using CsA, (G1b:7.5, G2b:15, and G3b: 30 mg/kg/day) at the first 13 minutes of reperfusion period. After 10 days pretreatment, the rat hearts were isolated and transferred to Langendorff apparatus and exposed to 30 minutes ischemia following 60 minutes reperfusion. Afterward, the hearts were preserved in 10% formalin for histological studies at the end of the experiment. Finally, hematoxylin and eosin and Masson's trichrome staining techniques were used for evaluating the changes in myocardial architecture, degradation of myofibers, and collagen integrity. The differences were analyzed using Pearson test.

RESULTS

Cell degenerative changes, pyknotic nuclei, contraction bands, edema, and loosening of collagen in between muscle fibers were observed during ischemia-reperfusion. Myocardial architecture and cellular morphology were recovered in co-administration groups, especially in (Gallic acid 15 mg/kg + CsA, P < 0.001).

CONCLUSIONS

The results suggest the important role of the antioxidant system potentiation in the prevention of myocardial damage.

摘要

背景

冠状动脉闭塞20分钟后开始出现不可逆的心肌缺血损伤。然后梗死细胞显示出坏死和死亡的迹象。

目的

本研究调查了将没食子酸(抗氧化剂)与环孢素(线粒体通透性转换孔 [mPTP] 抑制剂)联合使用对大鼠缺血再灌注期间心肌形态的影响。

材料与方法

54只雄性Wistar大鼠(250 - 300克),随机分为9组:假手术组、对照组(Ca组接受生理盐水,1毫升/千克,Cb组:用0.2微摩尔/升的环孢素CsA灌注),3组用生理盐水预处理没食子酸(G1a组:7.5、G2a组:15和G3a组:30毫克/千克/天,每天灌胃10天,n = 6),另外三组先用没食子酸预处理,然后在再灌注期的前13分钟用CsA灌注(G1b组:7.5、G2b组:15和G3b组:30毫克/千克/天)。预处理10天后,分离大鼠心脏并转移至Langendorff装置,在60分钟再灌注后进行30分钟缺血。之后,在实验结束时将心脏保存在10%福尔马林中用于组织学研究。最后,使用苏木精和伊红染色以及Masson三色染色技术评估心肌结构的变化、肌纤维的降解和胶原蛋白的完整性。使用Pearson检验分析差异。

结果

在缺血再灌注期间观察到细胞退行性变化、核固缩、收缩带、水肿以及肌纤维间胶原蛋白的疏松。联合给药组的心肌结构和细胞形态得到恢复,尤其是在(没食子酸15毫克/千克 + CsA组,P < 0.001)。

结论

结果表明抗氧化系统增强在预防心肌损伤中具有重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6029/4302406/af7ce3235a66/jjnpp-09-04-17186-g001.jpg

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