使用MonoSpin® C18萃取柱和液相色谱/串联质谱法快速同时测定人血清中的乙哌立松、托哌酮和替扎尼定。

Rapid simultaneous determination of eperisone, tolperisone, and tizanidine in human serum by using a MonoSpin® C18 extraction column and liquid chromatography/tandem mass spectrometry.

作者信息

Miura Naoya, Saito Takeshi, Taira Takayuki, Yamagiwa Takeshi, Morita Sein, Inokuchi Sadaki

出版信息

J AOAC Int. 2014 Nov-Dec;97(6):1546-51. doi: 10.5740/jaoacint.13-295.

Abstract

A method was developed for rapid toxicological analysis of eperisone, tolperisone, and tizanidine in human serum using a MonoSpin® C18 extraction column and LC/MS/MS. The method was validated for LOD, linearity, precision, and extraction recovery. This method was rapid with an LOD of 0.5 ng/mL, linearity range 1-500.0 ng/mL (r2 = 0.999), and RSD value below 14.6%. Extraction recovery from the sample was greater than 98.6, 98.8, and 88.5% for eperisone, tolperisone, and tizanidine, respectively. Results showed that combination of the MonoSpin C18 extraction column and LC/MS/MS is a simple and rapid method for the analysis of these three analytes, and a method is described for simultaneous quantitative determination of the analytes in human serum by LC/MSIMS. This method was used to determine the serum levels of eperisone in a patient with eperisone poisoning, and could be successfully applied for screening analyses in clinical cases other than poisoning.

摘要

建立了一种使用MonoSpin® C18萃取柱和LC/MS/MS对人血清中乙哌立松、托哌立松和替扎尼定进行快速毒理学分析的方法。该方法在检测限、线性、精密度和萃取回收率方面得到了验证。该方法快速，检测限为0.5 ng/mL，线性范围为1 - 500.0 ng/mL（r2 = 0.999），相对标准偏差值低于14.6%。乙哌立松、托哌立松和替扎尼定从样品中的萃取回收率分别大于98.6%、98.8%和88.5%。结果表明，MonoSpin C18萃取柱和LC/MS/MS的组合是分析这三种分析物的简单快速方法，并且描述了一种通过LC/MSIMS同时定量测定人血清中分析物的方法。该方法用于测定乙哌立松中毒患者血清中乙哌立松的水平，并可成功应用于中毒以外的临床病例的筛查分析。