水通道蛋白7在白色脂肪细胞中的动态亚细胞定位
Dynamic subcellular localization of aquaporin-7 in white adipocytes.
作者信息
Miyauchi Takayuki, Yamamoto Hiroyuki, Abe Yoichiro, Yoshida Go J, Rojek Aleksandra, Sohara Eisei, Uchida Shinichi, Nielsen Søren, Yasui Masato
机构信息
Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
出版信息
FEBS Lett. 2015 Feb 27;589(5):608-14. doi: 10.1016/j.febslet.2015.01.025. Epub 2015 Jan 30.
Aquaporin-7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7-null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re-localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI-58). This relocation was confirmed by functional studies in 3T3-L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes.
水通道蛋白7（AQP7）在脂肪组织中表达，可被水和甘油透过，并参与脂质代谢。AQP7基因敲除小鼠会出现肥胖、胰岛素抵抗和脂肪细胞肥大。在此，我们表明AQP7在脂肪细胞质膜中表达，并在小鼠白色脂肪组织中响应儿茶酚胺而重新定位于细胞内膜。我们发现AQP7的内化是由蛋白激酶A（PKA）激活和比较基因识别58（CGI-58）诱导的。这种重新定位在3T3-L1脂肪细胞的功能研究中得到了证实。总的来说，这些结果表明AQP7在皮质膜和细胞内膜中对脂肪细胞代谢都有多种作用。
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