Trounce I, Byrne E, Marzuki S
Department of Neurology, St Vincent's Hospital, Victoria, Australia.
Lancet. 1989 Mar 25;1(8639):637-9. doi: 10.1016/s0140-6736(89)92143-0.
State III (activated) mitochondrial respiration rates with pyruvate/malate, glutamate/malate, and succinate as substrates were assayed in isolated intact skeletal muscle mitochondria in 29 subjects aged 16-92 years. There was a significant negative correlation between respiration rate and age with all substrates tested. A similar trend was seen for respiratory enzyme activities assayed in muscle homogenate. These findings suggest a substantial fall in mitochondrial oxidative capacity in ageing muscle, which may contribute to reduced exercise capacity in elderly people. Mitochondrial respiratory failure may contribute to the ageing process in other organs.
在29名年龄在16至92岁的受试者的分离完整骨骼肌线粒体中，以丙酮酸/苹果酸、谷氨酸/苹果酸和琥珀酸为底物测定了状态III（激活的）线粒体呼吸速率。在所测试的所有底物中，呼吸速率与年龄之间存在显著的负相关。在肌肉匀浆中测定的呼吸酶活性也观察到类似趋势。这些发现表明，衰老肌肉中线粒体氧化能力大幅下降，这可能导致老年人运动能力降低。线粒体呼吸衰竭可能在其他器官的衰老过程中起作用。
