Sapmaz Ali, Ulus A Tulga, Turan Nilüfer N, Kaymaz F Figen, Yazıcıoğlu Hija, Ersöz Siyar, Simsek Erdal, Köksoy Cüneyt
Department of General Surgery, University of Ankara, Ankara, Turkey.
Cardiovascular Surgery Clinic, Turkiye Yuksek Ihtisas Hospital, Ankara, Turkey Cardiovascular Surgery Department, Hacettepe University, Ankara, Turkey
Vascular. 2015 Dec;23(6):614-21. doi: 10.1177/1708538114568702. Epub 2015 Feb 2.
This study was designed to test the effects of different types of preconditioning and postconditioning methods on spinal cord protection following aortic clamping.
The animals (rabbits) were divided into sham-operated, ischemic preconditioning, remote ischemic preconditioning, simultaneous aortic and ischemic remote preconditioning, and ischemic postconditioning groups. After neurological evaluations, ultrastructural analysis and immunohistochemical staining for caspase-3 were evaluated after 24 h following ischemia.
The neurological outcomes of the remote ischemic preconditioning (4.2 ± 0.4) and ischemic postconditioning (4.6 ± 0.8) groups were significantly improved when compared with the ischemia group (2.2 ± 04). The immunohistochemical analysis revealed that the lowest percentage of apoptosis was in-group ischemic preconditioning at 12.5 ± 30.6%. In the comparison of intracellular edema in an ultrastructural analysis, the ischemic preconditioning and ischemic postconditioning groups had significantly lower values than the ischemia group.
The conditioning methods attenuate ischemia-reperfusion injury for spinal cord injury. Ischemic and remote preconditioning and also postconditioning methods are simple to perform and inexpensive.
本研究旨在测试不同类型的预处理和后处理方法对主动脉钳夹后脊髓保护的影响。
将动物（兔子）分为假手术组、缺血预处理组、远程缺血预处理组、主动脉与缺血远程同步预处理组和缺血后处理组。在进行神经学评估后，于缺血24小时后评估超微结构分析和半胱天冬酶-3的免疫组织化学染色。
与缺血组（2.2±0.4）相比，远程缺血预处理组（4.2±0.4）和缺血后处理组（4.6±0.8）的神经学结果有显著改善。免疫组织化学分析显示，凋亡百分比最低的是缺血预处理组，为12.5±3.06%。在超微结构分析中对细胞内水肿进行比较时，缺血预处理组和缺血后处理组的值明显低于缺血组。
这些预处理和后处理方法可减轻脊髓损伤的缺血再灌注损伤。缺血预处理、远程预处理以及后处理方法操作简单且成本低廉。
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