豚鼠上丘切片中长时程增强的形成。

Formation of long-term potentiation in superior colliculus slices from the guinea pig.

作者信息

Okada Y, Miyamoto T

机构信息

Department of Physiology, School of Medicine, Kobe University, Japan.

出版信息

Neurosci Lett. 1989 Jan 2;96(1):108-13. doi: 10.1016/0304-3940(89)90251-6.

DOI:10.1016/0304-3940(89)90251-6

PMID:2564648

Abstract

Superior colliculus slices of sagittal section were prepared from the guinea pig. Postsynaptic potential (PSP) was evoked in the superficial grey layer (SGL) after the electrical stimulation to optic layer. Tetanic stimulation to the optic layer elicited long-term potentiation (LTP) in the PSP of the SGL. Tetanic stimulation of 20 s in duration and 50 Hz in frequency was most effective for the formation of LTP. The LTP formation was masked during application of 2-amino-5-phosphonovalerate (APV), a specific antagonist for N-methyl-D-aspartate (NMDA) receptor, but LTP was observed when APV was removed from the perfusion medium.

摘要

从豚鼠制备矢状切片的上丘。对视层进行电刺激后，在浅灰层（SGL）诱发突触后电位（PSP）。对视层进行强直刺激可在SGL的PSP中诱发长时程增强（LTP）。持续20秒、频率50赫兹的强直刺激对LTP的形成最有效。在应用N-甲基-D-天冬氨酸（NMDA）受体的特异性拮抗剂2-氨基-5-磷酸戊酸（APV）期间，LTP的形成被掩盖，但当从灌注培养基中去除APV时，可观察到LTP。