Ishimoto K S, Lory S
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.
Proc Natl Acad Sci U S A. 1989 Mar;86(6):1954-7. doi: 10.1073/pnas.86.6.1954.
The promoter region of the Pseudomonas aeruginosa pilin gene has a high degree of similarity to the nitrogen-regulated promoters of enteric bacteria. These promoters are recognized by the alternative sigma factor of RNA polymerase, termed RpoN (NtrA or GlnF). This observation suggested that the P. aeruginosa pilin gene may be transcribed by the RpoN-containing RNA polymerase. We, therefore, cloned the RpoN gene from P. aeruginosa into Escherichia coli (where it formed a functional product) and used that cloned gene to construct a mutant of P. aeruginosa that was insertionally inactivated in its RpoN gene. This mutant failed to synthesize pilin, indicating that the RpoN sigma factor is required for transcription of the pilin gene.
铜绿假单胞菌菌毛蛋白基因的启动子区域与肠道细菌的氮调节启动子高度相似。这些启动子可被RNA聚合酶的替代sigma因子识别，该因子称为RpoN（NtrA或GlnF）。这一观察结果表明，铜绿假单胞菌菌毛蛋白基因可能由含RpoN的RNA聚合酶转录。因此，我们将铜绿假单胞菌的RpoN基因克隆到大肠杆菌中（在那里它形成了一种功能性产物），并使用该克隆基因构建了一个铜绿假单胞菌突变体，其RpoN基因被插入失活。该突变体无法合成菌毛蛋白，表明菌毛蛋白基因的转录需要RpoN sigma因子。
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