在氮素充足和缺乏条件下，温度、pH 值和光照对钝形栅藻光合作用的影响。
Effect of the temperature, pH and irradiance on the photosynthetic activity by Scenedesmus obtusiusculus under nitrogen replete and deplete conditions.
机构信息
Doctorado en Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Mexico.
Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Mexico.
出版信息
Bioresour Technol. 2015 Apr;181:128-35. doi: 10.1016/j.biortech.2015.01.034. Epub 2015 Jan 17.
This paper evaluates the effect of the irradiance, pH and temperature on the photosynthetic activity (PA) of Scenedesmus obtusiusculus under N-replete and N-deplete conditions through oxygen measurements. The highest PA values were 160 mgO2 gb(-1) h(-1) at 620 μmol m(-2) s(-1), 35 °C and pH of 8 under N-replete conditions and 3.3 mgO2 gb(-1) h(-1) at 100 μmol m(-2) s(-1), 28.5 °C and pH of 5.5 for N-deplete conditions. Those operation conditions were tested in a flat-panel photobioreactor. The biomass productivity was 0.97 gb L(-1) d(-1) under N-replete conditions with a photosynthetic efficiency (PE) of 4.4% yielding 0.85 gb mol photon(-1). Similar biomass productivity was obtained under N-deplete condition; and the lipid productivity was 0.34 gL L(-1) d(-1) with a PE of 7.8% yielding 0.39 gL mol photon(-1). The apparent activation and deactivation energies were 16.1 and 30 kcal mol(-1), and 11.9 and 15.3 kcal mol(-1), for N-replete and N-deplete conditions, respectively.
本文通过氧测量评估了辐照度、pH 值和温度对 N 充足和 N 缺乏条件下钝顶节旋藻光合作用（PA）的影响。在 N 充足条件下，最高 PA 值为 620 μmol m(-2) s(-1)、35°C 和 pH 值 8 时为 160 mgO2 gb(-1) h(-1)，在 N 缺乏条件下，最高 PA 值为 100 μmol m(-2) s(-1)、28.5°C 和 pH 值 5.5 时为 3.3 mgO2 gb(-1) h(-1)。这些操作条件在平板光生物反应器中进行了测试。在 N 充足条件下，生物量生产力为 0.97 gb L(-1) d(-1)，光合效率（PE）为 4.4%，产生 0.85 gb mol 光子(-1)。在 N 缺乏条件下也获得了类似的生物量生产力；脂质生产力为 0.34 gL L(-1) d(-1)，PE 为 7.8%，产生 0.39 gL mol 光子(-1)。N 充足和 N 缺乏条件下的表观活化能和失活能分别为 16.1 和 30 kcal mol(-1)，以及 11.9 和 15.3 kcal mol(-1)。
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