Wang Meijian, Wang Yaqi, Zhong Jun
Department of Oncology, Jiangxi Provincial Tumor Hospital, Nanchang, Jiangxi 330029, P.R. China.
Mol Med Rep. 2015 Jun;11(6):4297-302. doi: 10.3892/mmr.2015.3291. Epub 2015 Jan 30.
Several studies have demonstrated that the isolated side population (SP) cells from solid tumors exhibit cancer stem cell‑like properties, and are responsible for drug resistance during chemotherapy and tumor recurrence. In the current study, cancer stem cell‑like SP cells were isolated in the MDU‑22 breast cancer cell line using the Hoechst‑33342 dye exclusion technique. It was observed that SP cells accounted for 3.8% of cells in the MDU‑22 cell line, which was reduced to 0.6% in the presence of verapamil, an inhibitor of the ABC transporter. The sorted SP cells showed an elevated expression of stem cell markers, including ABCG2, OCT‑4 and EpCAM. Furthermore, it was demonstrated that the isolated SP cells undergo rapid proliferation and have a high survival rate. These results indicate that the coexpression of adenosine triphosphatase binding cassette transporters and stem cell surface markers in SP cells may contribute to chemoresistance, tumor recurrence, metastasis and invasion. Therefore, the isolation and characterization of SP cells may provide novel insights for the development of alternative therapeutic agents to target cancer stem cells.
多项研究表明，从实体瘤中分离出的侧群（SP）细胞具有癌症干细胞样特性，并且在化疗期间导致耐药性以及肿瘤复发。在本研究中，使用Hoechst-33342染料排除技术在MDU-22乳腺癌细胞系中分离出癌症干细胞样SP细胞。观察到SP细胞占MDU-22细胞系中细胞的3.8%，在存在ABC转运蛋白抑制剂维拉帕米的情况下，这一比例降至0.6%。分选的SP细胞显示出干细胞标志物的表达升高，包括ABCG2、OCT-4和EpCAM。此外，已证明分离出的SP细胞经历快速增殖并且具有高存活率。这些结果表明，SP细胞中三磷酸腺苷结合盒转运蛋白与干细胞表面标志物的共表达可能导致化疗耐药、肿瘤复发、转移和侵袭。因此，SP细胞的分离和表征可能为开发靶向癌症干细胞的替代治疗药物提供新的见解。
