Mahfouz Magdy Elsayed, Mira Nabila, Amer Said
Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr El Sheikh, 33516, Egypt.
J Vet Med Sci. 2014 Dec;76(12):1569-75. doi: 10.1292/jvms.14-0272. Epub 2014 Sep 2.
In this study, we examined the prevalence and molecular characteristics of Cryptosporidium in buffalo, dairy cattle and sheep in different farms at Kafr El Sheikh Province, Egypt. Rectal fecal samples, including 466 samples from buffalo, 1697 from cattle and 120 from sheep, were collected from different ages and screened by modified Ziehl-Neelsen acid-fast microscopy for detection of Cryptosporidium oocysts. All studied farms were positives with an overall prevalence of 1.29% in buffalo (4.17% in claves versus 0.48% in adults), 7.07% in cattle (6.90% in calves versus 10.20% and 6.10% in heifers and adults, respectively) and 2.50% in sheep (4.40% in lambs versus 1.30% in adults). PCR-RFLP analyses of small-subunit rRNA genes from positive specimens revealed the occurrence of C. parvum and C. ryanae in buffalo; C. parvum, C. ryanae, C. bovis and C. andersoni in cattle and only C. xiaoi in sheep. Genotypes distribution showed that C. ryanae was the dominant species (60.0%) followed by C. parvum (40.0%) in buffalo calves. Meanwhile, in cattle calves, C. parvum was the commonest species (74.23%) followed by C. ryanae (16.10%) and C. bovis (9.70%). Subtyping of C. parvum based on sequence analysis of the polymorphic 60 kDa glycoprotein gene locus showed the presence of subtypes IIdA20G1 and IIaA15G1R1 in both buffalo and cattle calves, addressing the potential role of calves in zoonotic cryptosporidiosis in Egypt.
在本研究中，我们调查了埃及卡夫尔谢赫省不同农场的水牛、奶牛和绵羊隐孢子虫的流行情况及分子特征。采集了不同年龄段的直肠粪便样本，包括466份水牛样本、1697份奶牛样本和120份绵羊样本，并通过改良齐尔-尼尔森抗酸显微镜检查法筛选隐孢子虫卵囊。所有研究农场均呈阳性，水牛的总体患病率为1.29%（犊牛为4.17%，成年牛为0.48%），奶牛为7.07%（犊牛为6.90%，小母牛和成年牛分别为10.20%和6.10%），绵羊为2.50%（羔羊为4.40%，成年羊为1.30%）。对阳性样本的小亚基rRNA基因进行PCR-RFLP分析，结果显示水牛体内存在微小隐孢子虫和瑞氏隐孢子虫；奶牛体内存在微小隐孢子虫、瑞氏隐孢子虫、牛隐孢子虫和安氏隐孢子虫；绵羊体内仅存在肖氏隐孢子虫。基因型分布表明，水牛犊牛中瑞氏隐孢子虫是优势种（60.0%），其次是微小隐孢子虫（40.0%）。同时，在奶牛犊牛中，微小隐孢子虫是最常见的种类（74.23%），其次是瑞氏隐孢子虫（16.10%）和牛隐孢子虫（9.70%）。基于多态性60 kDa糖蛋白基因座序列分析的微小隐孢子虫亚型分型显示，水牛和奶牛犊牛中均存在IIdA20G1和IIaA15G1R1亚型，这表明犊牛在埃及人兽共患隐孢子虫病中可能发挥作用。
