Yang Falong, Dao Xiaofang, Rodriguez-Palacios Alex, Feng Xufei, Tang Cheng, Yang Xiaonong, Yue Hua
Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China.
J Vet Med Sci. 2014 Dec;76(12):1631-4. doi: 10.1292/jvms.14-0094. Epub 2014 Aug 26.
A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ovipneumoniae DNA over a wide linear range, from 2.2 × 10(2) to 2.2 × 10(7) genomes.
利用9个最近测序的绵羊肺炎支原体基因组和靶向假定粘附素基因p113的引物，开发了一种用于检测和定量绵羊肺炎支原体的实时PCR方法。该检测方法被证明具有特异性和敏感性（检测限为22个基因组DNA），并且能够在2.2×10²至2.2×10⁷个基因组的宽线性范围内定量绵羊肺炎支原体DNA。
