Nishine Kaoru, Aoki Hiroshi, Sakoda Yoshihiro, Fukusho Akio
School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
J Vet Med Sci. 2014 Dec;76(12):1635-9. doi: 10.1292/jvms.14-0220. Epub 2014 Sep 2.
Field isolates of BVDV which do not show the exaltation of Newcastle disease virus (END) phenomenon (END(-)) are rarely reported. In this study, 45 BVDV field isolates from cattle in Hokkaido prefecture in Japan were analyzed by the reverse plaque formation method, the END method and observation of cytopathic effects. END(-) virus was detected in 34 of 45 isolates (75.6%), although 35 of 45 field isolates contained END phenomenon positive virus as the predominant virus population. We propose that END(-) viruses are widely distributed in the field and that it is possible that the mixture of biologically distinct BVDV correlates with the appearance of disease in infected animals.
未表现出新城疫病毒强化（END）现象（END(-)）的牛病毒性腹泻病毒（BVDV）野外分离株鲜有报道。在本研究中，采用反向蚀斑形成法、END法及细胞病变效应观察，对来自日本北海道地区牛的45株BVDV野外分离株进行了分析。45株分离株中有34株（75.6%）检测到END(-)病毒，尽管45株野外分离株中有35株含有以END现象阳性病毒为主的病毒群体。我们提出，END(-)病毒在野外广泛分布，并且生物学特性不同的BVDV混合可能与感染动物疾病的出现有关。
