Pahangchopi Donna, Singh Ran Vir, Singh Shoor Vir, Das Paritosh, Sharma Deepak, Sardana Tarun, Kumar Naveen, Chaubey Kundan Kumar, Gupta Saurabh
Indian J Exp Biol. 2014 Dec;52(12):1182-5.
Two antigens ('cattle' type and 'Indian Bison' type) of Mycobacterium avium subspecies paratuberculosis were evaluated for diagnosis of Johne's disease (JD) in a gaushala (cattle herd). Of the 160 cows of Sahiwal and Hariana breeds screened, 81 (50.6%) tested positive in ELISA and 66 (41.8%) in AGPT test. Using the two antigens, 33.5% tested positive in both the tests while 41.1% tested negative. Exclusively, only 8.2% tested positive in ELISA while 17.1% tested positive in AGPT. Two antigens together detected 58.9% prevalence of MAP in the gaushala. Individually, indigenous ELISA using antigen from native source of MAP proved superior to AGPT in the diagnosis of JD in cows.
对副结核分枝杆菌的两种抗原（“牛”型和“印度野牛”型）进行了评估，以诊断一个奶牛场（牛群）中的副结核病（JD）。在筛查的160头萨希瓦尔和哈里亚纳品种奶牛中，81头（50.6%）ELISA检测呈阳性，66头（41.8%）琼脂凝胶沉淀试验（AGPT）检测呈阳性。使用这两种抗原时，两项检测均呈阳性的占33.5%，而检测呈阴性的占41.1%。单独来看，ELISA检测呈阳性的仅占8.2%，AGPT检测呈阳性的占17.1%。两种抗原一起检测出该奶牛场中副结核分枝杆菌（MAP）的流行率为58.9%。就个体而言，使用来自MAP本地来源抗原的本土ELISA在奶牛副结核病诊断方面优于AGPT。
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