Donald J A, Lillywhite H B
Department of Zoology, University of Florida, Gainesville 32611.
Cell Tissue Res. 1989 Mar;255(3):585-8. doi: 10.1007/BF00218794.
The indirect immunofluorescence technique was used to determine the distribution of vasoactive intestinal polypeptide-immunoreactive and somatostatin-immunoreactive axons in the pulmonary vasculature of the aquatic file snake Acrochordus granulatus. A dense distribution of vasoactive intestinal polypeptide-immunoreactive axons was found on the common pulmonary artery, the anterior and posterior pulmonary arteries, and the smaller arteries branching to the lung. The density of these axons appeared greater in arterial preparations taken from more distal regions of the lung. The densest distribution of vasoactive intestinal polypeptide-immunoreactive axons was observed on the larger pulmonary veins in all regions of the lung. These axons were observed on the larger veins within the lung parenchyma but not on the smaller veins. Axons and cell bodies were observed in the vagal nerve trunks which run parallel to the pulmonary arteries and veins. In contrast, no somatostatin-immunoreactive axons were observed in any region of the pulmonary vasculature. It is proposed that the perivascular plexus of vasoactive intestinal polypeptide-immunoreactive axons may represent part or all of the vagal postganglionic innervation of the pulmonary vasculature.
采用间接免疫荧光技术，确定血管活性肠肽免疫反应性轴突和生长抑素免疫反应性轴突在粒棱蛇肺血管系统中的分布。在肺动脉主干、左右肺动脉以及分支至肺的较小动脉上，发现有密集分布的血管活性肠肽免疫反应性轴突。取自肺更远端区域的动脉标本中，这些轴突的密度似乎更高。在肺所有区域的较大肺静脉上，观察到血管活性肠肽免疫反应性轴突分布最为密集。在肺实质内的较大静脉上观察到了这些轴突，但在较小静脉上未观察到。在与肺动脉和肺静脉平行走行的迷走神经干中观察到了轴突和细胞体。相比之下，在肺血管系统的任何区域均未观察到生长抑素免疫反应性轴突。有人提出，血管活性肠肽免疫反应性轴突的血管周围神经丛可能代表了肺血管系统迷走神经节后神经支配的部分或全部。
