Ballanyi K, Dörner R, Schlue W R
Physiologisches Institut, Zentrum Physiologie und Pathophysiologie, Georg-August-Universität, Göttingen, Federal Republic of Germany.
Glia. 1989;2(1):51-4. doi: 10.1002/glia.440020106.
Na+-selective, double-barrelled microelectrodes were used to measure intracellular Na+ activity (aiNa) and membrane potential (Em) in neuropile glial cells of isolated segmental ganglia in the leech Hirudo medicinalis. Bath application of glutamate (10(-3) M) resulted in membrane depolarizations of about 5 mV and a concomitant increase of aiNa by between 2 and 10 mM. Kainate (10(-4) M) elicited depolarizations of up to 40 mV amplitude followed by a prominent after hyperpolarization. During kainate, aiNa increased by 7 to 25 mM. In contrast to glutamate, an initial decrease of aiNa was detected during the action of kainate. N-methyl-D-aspartate (NMDA, 10(-5)-10(-3) M) had no effect of Em and aiNa. The results indicate that leech glial cells have a kainate-preferring non-NMDA glutamate receptor.
使用钠选择性双管微电极来测量药用水蛭离体节段神经节神经毡胶质细胞内的钠活性（aiNa）和膜电位（Em）。浴槽中加入谷氨酸（10⁻³ M）会导致膜去极化约5 mV，并伴随aiNa增加2至10 mM。 kainate（10⁻⁴ M）引起高达40 mV幅度的去极化，随后是明显的超极化后电位。在kainate作用期间，aiNa增加7至25 mM。与谷氨酸不同，在kainate作用期间检测到aiNa最初有所下降。 N-甲基-D-天冬氨酸（NMDA，10⁻⁵ - 10⁻³ M）对Em和aiNa没有影响。结果表明，水蛭胶质细胞具有对kainate优先的非NMDA型谷氨酸受体。
