基于牧草的绵羊日粮添加生大蒜、大蒜油和莫能菌素后的体外消化率评估。
In vitro assessment of the digestibility of forage based sheep diet, supplemented with raw garlic, garlic oil and monensin.
作者信息
Anassori Ehsan, Dalir-Naghadeh Bahram, Pirmohammadi Rasoul, Taghizadeh Akbar, Asri-Rezaei Siamak, Farahmand-Azar Safa, Besharati Maghsoud, Tahmoozi Morteza
机构信息
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran;
出版信息
Vet Res Forum. 2012 Winter;3(1):5-11.
The effect of inclusion of garlic essential oil (EO) at 33, 66 and 100 µg mL(-1), raw garlic (GAR) at 5, 10 and 15 mg mL(-1) and monensin (MON) at 7.5 µg mL(-1) of incubation medium on organic matter digestibility (OMD) was studied with in vitro gas production, ANKOM daisy(II) and conventional in vitro (IVOMD) methods. The material was incubated with sheep ruminal fluid and the experimental design was a completely randomized design. Cumulative gas production was recorded at 0, 2, 4, 6, 8, 12, 16, 24, 36, 48, 72 and 96 hours of incubation. Conventional in vitro OMD was determined after 48 hours incubation in acid and pepsin solutions. Samples for Daisy(II) OMD prepared according to the operating instructions supplied by ANKOM(®) Tech. Co., Fairport, USA. Compared to in vitro dry matter digestibility (IVDMD), Daisy(II) and gas production techniques overestimated (P < 0.05) OMD. The addition of EO and MON reduced (P < 0.05) the organic matter (OM), neutral detergent fiber (NDF), acid-detergent fiber (ADF) digestion, and gas production volume. The potential of gas production and rate of gas production for EO and MON were the lowest; however, these variables were higher for GAR supplemented groups. It was concluded that raw garlic could be of great interest for its usage as a modulator of ruminal fermentation.
采用体外产气法、ANKOM daisy（II）法和常规体外法（IVOMD），研究了在培养基中添加33、66和100 µg mL⁻¹大蒜精油（EO）、5、10和15 mg mL⁻¹生大蒜（GAR）以及7.5 µg mL⁻¹莫能菌素（MON）对有机物消化率（OMD）的影响。将材料与绵羊瘤胃液一起培养，实验设计为完全随机设计。在培养0、2、4、6、8、12、16、24、36、48、72和96小时时记录累积产气量。常规体外OMD在酸和胃蛋白酶溶液中培养48小时后测定。根据美国费尔波特ANKOM（®）技术公司提供的操作说明制备Daisy（II）OMD的样品。与体外干物质消化率（IVDMD）相比，Daisy（II）法和产气技术高估了（P < 0.05）OMD。添加EO和MON降低了（P < 0.05）有机物（OM）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）的消化率以及产气量。EO和MON的产气潜力和产气速率最低；然而，这些变量在添加GAR的组中较高。得出的结论是，生大蒜作为瘤胃发酵调节剂的用途可能非常有意义。
Zotero 插件