Kaur Kulbir, Simon Anne F, Chauhan Ved, Chauhan Abha
Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA; Biology/Neuroscience Graduate Program, City University of New York - Graduate Center, 365 5th Avenue, New York, NY 10016, USA; Center for Developmental Neuroscience and Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA.
Department of Biology, Faculty of Science, Western Ontario University, Ontario, Canada.
Behav Brain Res. 2015 May 1;284:77-84. doi: 10.1016/j.bbr.2015.02.001. Epub 2015 Feb 7.
Developmental disorders such as autism and attention deficit hyperactivity disorder (ADHD) appear to have a complex etiology implicating both genetic and environmental factors. Bisphenol A (BPA), a widely used chemical in the plastic containers and in the linings of food and beverage cans, has been suggested to play a possible causative role in some developmental disorders. Here, we report behavioral modifications in Drosophila melanogaster following early exposure to BPA, which may suggest BPA as an environmental risk factor for the behavioral impairments that are the basis of diagnosis of autism and ADHD. In an open field assay with perinatally BPA-exposed and vehicle-treated control Drosophila, different parameters of locomotion (distance traveled, walking speed, spatial movement, mobility, turn angle, angular velocity and meander) were analyzed using the ethovision software. We also examined the repetitive and social interaction behaviors in these flies. In an open field assay, we identified disturbances in the locomotion patterns of BPA-exposed Drosophila that may relate to the decision-making and the motivational state of the animal. An increase in repetitive behavior was observed as an increase in the grooming behavior of Drosophila following BPA exposure. Furthermore, we also observed abnormal social interaction by the BPA-exposed flies in a social setting. These results demonstrate the effect of the environmentally prevalent risk agent BPA on the behavior of Drosophila, and suggest the practicability and the ease of using Drosophila as a model in the studies of neurobehavioral developmental disorders.
自闭症和注意力缺陷多动障碍（ADHD）等发育障碍似乎具有复杂的病因，涉及遗传和环境因素。双酚A（BPA）是一种广泛用于塑料容器以及食品和饮料罐内衬的化学物质，有人认为它可能在某些发育障碍中起致病作用。在此，我们报告了黑腹果蝇在早期接触BPA后的行为改变，这可能表明BPA是导致自闭症和ADHD诊断所依据的行为障碍的环境风险因素。在对围产期接触BPA和用载体处理的对照果蝇进行的旷场试验中，使用EthoVision软件分析了不同的运动参数（行进距离、行走速度、空间移动、移动性、转弯角度、角速度和曲折度）。我们还检查了这些果蝇的重复行为和社交互动行为。在旷场试验中，我们发现接触BPA的果蝇的运动模式受到干扰，这可能与动物的决策和动机状态有关。观察到重复行为增加，表现为BPA暴露后果蝇梳理行为的增加。此外，我们还观察到接触BPA的果蝇在社交环境中的社交互动异常。这些结果证明了环境中普遍存在的风险因素BPA对果蝇行为的影响，并表明使用果蝇作为神经行为发育障碍研究模型的实用性和便利性。
