Lei Chun-Tao, Wu Xiao-Ling, Peng Jie, Chen Xiao-Feng, Qiao Li-Feng, Fan Ying-Chuan, Hu Jian-Bin
Department of Ophthalmology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, School of Clinical Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, China.
Department of Digestion, Chengdu Military General Hospital, Chengdu, 610083, China.
J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):135-139. doi: 10.1007/s11596-015-1402-9. Epub 2015 Feb 12.
The effects of the balance changes of pigment epithelium growth factor (PEDF) and vascular endothelial growth factor (VEGF) in whole-body and retinal tissue on rats with oxygen-induced retinopathy were investigated. Forty-eight neonatal SD rats at the age of 7 days were randomly divided into 4 groups. The neonatal rats in experimental groups were exposed to 75% to 80% oxygen for 5 days and then to normal air, and those in control groups were kept feeding in normal air. At the age of 17 and 22 days, all the neonatal rats received retina angiography with FITC-dextran and the pathological changes of retinal vessels and perfusion were observed. HE staining of the tissue section and the number counting of endothelial cells extending beyond the inner limiting membrane were performed to evaluate the endothelial proliferation. Immunohistochemistry was applied to detect the expression of PEDF and VEGF in retinal tissue, and ELISA to detect their expression in serum. A hypoxic-ischemic proliferation of retina and more endothelial cells extending beyond the inner limiting membrane were found in the neonatal rats in both experimental groups of 17-day old and 22-day old as compared with those in control group with the difference being statistically significant (P<0.01). VEGF staining of the rats in the 17-day old experimental group was significantly stronger, with an increasing positive rate, than that of the rats in the 17-day old control group (P<0.01). PEDF staining of the rats of 22 days old was weaker than that of the rats of 17 days old in the experimental groups (P<0.01). There was no significant difference in serum VEGF concentration among all groups (P>0.05). The serum PEDF concentration in the rats of 17 days old in experimental group was decreased significantly as compared with that in the rats of 17 days old in control group (P<0.01), and in experimental groups, the serum PEDF concentration of the rats of 22 days old was increased as compared with that of the rats of 17 days old (P<0.01). In conclusion, the obviously decreased serum PEDF concentration and the abnormal enhanced expression of VEGF density in local retinal tissue broke down the balance of PEDF/VEGF in whole-body or local tissues, which might play an important role in retinal vascular proliferation.
研究色素上皮生长因子（PEDF）和血管内皮生长因子（VEGF）在全身及视网膜组织中的平衡变化对氧诱导视网膜病变大鼠的影响。将48只7日龄新生SD大鼠随机分为4组。实验组新生大鼠置于75%至80%氧气环境中5天，然后置于正常空气中，对照组新生大鼠一直饲养在正常空气中。在17日龄和22日龄时，所有新生大鼠接受异硫氰酸荧光素标记葡聚糖视网膜血管造影，观察视网膜血管的病理变化及灌注情况。对组织切片进行HE染色，并对延伸至内界膜以外的内皮细胞进行计数，以评估内皮细胞增殖情况。应用免疫组化检测视网膜组织中PEDF和VEGF的表达，应用酶联免疫吸附测定法检测血清中它们的表达。与对照组相比，17日龄和22日龄实验组新生大鼠均出现视网膜缺氧缺血性增殖，且延伸至内界膜以外的内皮细胞增多，差异有统计学意义（P<0.01）。17日龄实验组大鼠的VEGF染色明显强于17日龄对照组大鼠，阳性率增加（P<0.01）。实验组中22日龄大鼠的PEDF染色弱于17日龄大鼠（P<0.01）。各组血清VEGF浓度差异无统计学意义（P>0.05）。实验组17日龄大鼠血清PEDF浓度较对照组17日龄大鼠显著降低（P<0.01），且在实验组中，22日龄大鼠血清PEDF浓度较17日龄大鼠升高（P<0.01）。综上所述，血清PEDF浓度明显降低以及局部视网膜组织中VEGF密度异常增强表达，打破了全身或局部组织中PEDF/VEGF的平衡，这可能在视网膜血管增殖中起重要作用。
