Program in Fisheries Science, Division of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Bioresour Technol. 2015 Sep;191:377-84. doi: 10.1016/j.biortech.2015.01.091. Epub 2015 Feb 2.
This work aimed to find an optimum culture medium for green microalga Botryococcus braunii KMITL and investigate its biodiesel properties based on fatty acid composition. Four different media were tested. Chlorella medium was the best medium for lipid yield. Among four nitrogen sources tested, KNO3 produced the highest lipid yield. When varied the nitrogen concentrations, this strain gave the highest lipid yield at the highest nitrogen level. When cultivated in the best medium and nitrogen source and level for 30 days, and then cultivated further for 14 days in the medium with no nitrogen, the highest lipid content and yield were 49.94±0.82% and 2.71±0.02 g L(-1), respectively. C16:0 fatty acid was the major fatty acid found. Fatty acid profiles of B. braunii KMITL cultivated in Chlorella medium with 1.25 g L(-1) KNO3 gave the best biodiesel properties with the lowest iodine value, maximum cetane number, and lowest degree of unsaturation.
本工作旨在寻找最佳的培养基培养绿色微藻柏氏杜氏藻 KMITL，并根据脂肪酸组成研究其生物柴油特性。测试了四种不同的培养基。在四种氮源中，硝酸钾产生的脂质产量最高。当改变氮浓度时，该菌株在最高氮水平下产生最高的脂质产量。在最佳培养基和氮源及浓度下培养 30 天后，再在无氮培养基中培养 14 天，脂质含量和产率分别达到 49.94±0.82%和 2.71±0.02 g L(-1)。发现的主要脂肪酸是 C16:0 脂肪酸。在含有 1.25 g L(-1)硝酸钾的小球藻培养基中培养的柏氏杜氏藻 KMITL 的脂肪酸图谱具有最佳的生物柴油特性，碘值最低、十六烷值最高、不饱和程度最低。
