基于促性腺激素释放激素的公羊（绵羊）亚单位疫苗的设计、合成与评价
Design, synthesis and evaluation of a gonadotropin releasing hormone-based subunit vaccine in rams (Ovis aries).
作者信息
Goodwin Daryn, Varamini Pegah, Simerska Pavla, D'Occhio Michael J, Toth Istvan
机构信息
The University of Queensland, School of Chemistry and Molecular Biosciences, St. Lucia, Queensland 4072, Australia.
The University of Sydney, Faculty of Agriculture and Environment, Camden Campus, Camden, New South Wales 2570, Australia.
出版信息
Vaccine. 2015 Mar 17;33(12):1453-8. doi: 10.1016/j.vaccine.2015.01.076. Epub 2015 Feb 9.
Immunocastration using gonadotropin-releasing hormone (GnRH)-based vaccines has been investigated in rams to reduce aggressive and sexual behaviour and to control meat quality. Despite considerable efforts, a practical GnRH vaccine has yet to be developed for rams. In the present study, a A GnRH-lipopeptide vaccine (GnRH-LP) including two copies of GnRH, 2-amino-d,l-hexadecanoic acid (C16), and a unique T helper epitope, was examined in rams. Rams received a primary and secondary vaccination of GnRH-LP without additional adjuvant (Group 1) or with the adjuvant AdjuVac™ (Group 2). In both Group 1 and 2 anti-GnRH antibody titres increased after secondary vaccination, however, the antibody titres were higher (p<0.01) for rams in Group 2. The latter rams showed a marked decrease in testicular size. The marked and sustained reduction in testicular size in rams treated with GnRH-LP+AdjuVac™ provides the basis for an effective immunocastration vaccine in rams.
在公羊中已对使用基于促性腺激素释放激素（GnRH）的疫苗进行免疫去势进行了研究，以减少攻击性行为和性行为，并控制肉质。尽管付出了巨大努力，但尚未为公羊开发出实用的GnRH疫苗。在本研究中，对公羊检测了一种包含两个GnRH拷贝、2-氨基-d，l-十六烷酸（C16）和一个独特的T辅助表位的GnRH脂肽疫苗（GnRH-LP）。公羊接受了GnRH-LP的初次和二次疫苗接种，其中一组不添加额外佐剂（第1组），另一组添加佐剂AdjuVac™（第2组）。在第1组和第2组中，二次疫苗接种后抗GnRH抗体滴度均升高，然而，第2组公羊的抗体滴度更高（p<0.01）。后一组公羊的睾丸大小显著减小。用GnRH-LP+AdjuVac™处理的公羊睾丸大小显著且持续减小，这为开发一种有效的公羊免疫去势疫苗提供了基础。
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