假定的小鼠原始生殖细胞群体中的造血活性。

Hematopoietic activity in putative mouse primordial germ cell populations.

作者信息

Scaldaferri Maria Lucia, Klinger Francesca Gioia, Farini Donatella, Di Carlo Anna, Carsetti Rita, Giorda Ezio, De Felici Massimo

机构信息

Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.

Research Center Ospedale Pediatrico Bambino Gesù, IRCSS, Laboratory of Flow-Cytometry and B Cell Development, Rome, Italy.

出版信息

Mech Dev. 2015 May;136:53-63. doi: 10.1016/j.mod.2015.02.002. Epub 2015 Feb 12.

DOI:10.1016/j.mod.2015.02.002

PMID:25684074

Abstract

In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.

摘要

在本论文中，我们从观察定位于主动脉-性腺-中肾（AGM）区域的假定小鼠原始生殖细胞（PGC）群体中GOF-18ΔPE-GFP Pou5f1（Oct3/4）转基因的异质性表达开始，鉴定出了各种OCT3/4阳性群体，这些群体表现出PGC标志物（BLIMP-1、AP、TG-1、STELLA）的不同表达，并共表达造血前体细胞的几种蛋白质（CD-34、CD-41、FLK-1）和基因（Brachyury、Hox-B4、Scl/Tal-1和Gata-2）。此外，我们发现Oct3/4-GFP（弱阳性）CD-34（弱阳性/强阳性）细胞在体外具有强大的造血集落形成活性（CFU）。这些数据表明，通常被认为是向生殖嵴迁移的PGC的细胞群体包含了一个共表达几种生殖细胞和造血标志物并具有造血活性的细胞亚群。这些结果在当前的种系分离模型中进行了讨论。