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在电场和磁场下,能否用吕色萤光黄指示生物细胞膜的正确通透性?

Can Lucifer Yellow Indicate Correct Permeability of Biological Cell Membrane under An Electric and Magnetic Field?

机构信息

Department of Physics, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.

Department of Medical Physics, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.

出版信息

Cell J. 2015 Winter;16(4):560-3. doi: 10.22074/cellj.2015.501. Epub 2015 Jan 13.

Abstract

The effect of external magnetic and electric fields, in the range of electroporation and magnetoporation, on Lucifer Yellow (LY) fluorescence in the absence of cells is studied. Electric-field-induced quenching and magnetic field-induced increase are observed for fluorescence intensity of LY. Regard to the fact that the variation of field-induced fluorescence, even in the absence of cells, can be observed, the application of LY, as a marker, is debatable in electroporation and magnetoporation techniques.

摘要

研究了电穿孔和磁穿孔范围内的外加磁场和电场对无细胞状态下 Lucifer Yellow(LY)荧光的影响。观察到电场诱导的荧光猝灭和磁场诱导的荧光强度增加。鉴于即使在无细胞状态下也可以观察到场诱导荧光的变化,因此将 LY 用作标记物在电穿孔和磁穿孔技术中的应用存在争议。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/819d/4297495/ddc43e534059/Cell-J-16-560-g01.jpg

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