单颗粒水平下金纳米材料的自动计数

Automatic enumeration of gold nanomaterials at the single-particle level.

作者信息

Xu Xiao, Li Tian, Xu Zhongxing, Wei Hejia, Lin Ruoyun, Xia Bin, Liu Feng, Li Na

机构信息

Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University , Beijing, 100871, China.

出版信息

Anal Chem. 2015 Mar 3;87(5):2576-81. doi: 10.1021/ac503756f. Epub 2015 Feb 16.

DOI:10.1021/ac503756f

PMID:25686217

Abstract

In this study, we developed a highly sensitive automatic counting method for gold nanomaterials at the single particle level, which can serve as a general sensing platform based on counting of gold nanomaterials. This method substantially improved the sensitivity and accuracy for AuNP counting by adopting the color image processing based on the distinctive localized plasmonic light-scattering of gold nanomaterials. The 60-nm AuNPs, with concentrations down to 4 fM, can be detected with our method. As a universal counting approach for gold nanomaterials, such as gold nanospheres, nanorods, and aggregates from particles under detectable size, this quantification method should be versatile to a breadth of applications.

摘要

在本研究中，我们开发了一种用于单颗粒水平金纳米材料的高灵敏度自动计数方法，该方法可作为基于金纳米材料计数的通用传感平台。通过采用基于金纳米材料独特的局域表面等离子体光散射的彩色图像处理，该方法显著提高了金纳米颗粒计数的灵敏度和准确性。我们的方法能够检测浓度低至4 fM的60纳米金纳米颗粒。作为一种用于金纳米材料（如金纳米球、纳米棒以及可检测尺寸以下颗粒的聚集体）的通用计数方法，这种定量方法应广泛适用于多种应用。