Kanabagatte Basavarajappa Mallikarjuna, Song Haichen, Lamichhane Chinta, Samal Siba K
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, USA.
Zoetis, College Park, Maryland, USA.
J Clin Microbiol. 2015 May;53(5):1727-30. doi: 10.1128/JCM.02540-14. Epub 2015 Feb 18.
For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with vectored vaccine, using recombinant Newcastle disease virus (rNDV) expressing glycoprotein D (gD) as the vaccine vector.
为检测传染性喉气管炎病毒(ILTV)抗体,分别开发了基于糖蛋白B、C和D的酶联免疫吸附测定法(分别为B-ELISA、C-ELISA和D-ELISA)。与商业ELISA相比,B-ELISA和D-ELISA在检测感染鸡的抗ILTV抗体方面表现出更高的检测能力。此外,D-ELISA使用表达糖蛋白D(gD)的重组新城疫病毒(rNDV)作为疫苗载体,在检测载体疫苗诱导的血清转化方面效果良好。