Roy Nayan, Paul Pradip C, Singh T Sanjoy
Department of Chemistry, Assam University, Silchar, Assam 788 011, India.
Department of Chemistry, Assam University, Silchar, Assam 788 011, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 May 5;142:331-8. doi: 10.1016/j.saa.2015.02.002. Epub 2015 Feb 14.
Fluorescence properties of Schiff base - N,N'-bis(salicylidene) - 1,2-phenylenediamine (LH2) is used to study the micelles formed by aggregation of different important bile acids like cholic acid, deoxycholic acid, chenodeoxycholic acid and glycocholic acid by steady state and picosecond time-resolved fluorescence spectroscopy. The fluorescence band intensity was found out to increase with concomitant red shift with gradual addition of different bile acids. Binding constant of the probe with different bile acids as well as critical micelle concentration was obtained from the variation of fluorescence intensity on increasing concentration of bile acids in the medium. The increase in fluorescence quantum yields, fluorescence decay times and substantial decrease in nonradiative decay rate constants in bile acids micellar environment points to the restricted motion of the fluorophore inside the micellar subdomains.
席夫碱 - N,N'-双（水杨醛）-1,2 - 苯二胺（LH2）的荧光特性通过稳态和皮秒时间分辨荧光光谱法用于研究由不同重要胆汁酸如胆酸、脱氧胆酸、鹅去氧胆酸和甘胆酸聚集形成的胶束。发现随着不同胆汁酸的逐渐添加，荧光带强度增加并伴随红移。通过介质中胆汁酸浓度增加时荧光强度的变化，获得了探针与不同胆汁酸的结合常数以及临界胶束浓度。在胆汁酸胶束环境中荧光量子产率的增加、荧光衰减时间以及非辐射衰减速率常数的大幅降低表明荧光团在胶束亚域内的运动受到限制。
