Bednaski A V, Trevisan-Silva D, Matsubara F H, Boia-Ferreira M, Olivério M M, Gremski L H, Cavalheiro R P, De Paula D M B, Paredes-Gamero E J, Takahashi H K, Toledo M S, Nader H B, Veiga S S, Chaim O M, Senff-Ribeiro A
Department of Cell Biology, Federal University of Paraná, UFPR, Curitiba, PR, Brazil.
Department of Clinical Pathology, Clinical Hospital, Federal University of Paraná, Brazil.
Toxicon. 2015 May;98:62-74. doi: 10.1016/j.toxicon.2015.02.008. Epub 2015 Feb 24.
This is the first study on the hemolymph from a spider of the Loxosceles genus. These animals are responsible for a great number of envenomation cases worldwide. Several studies on Loxosceles venoms have been published, and the knowledge about the venom and its toxins is considerable, not only regarding the biological and biochemical characterization, but also regarding structural, genetic and phylogenetic approaches. However, the literature on Loxosceles hemolymph is nonexistent. The main goal of the present study was to characterize biochemically the hemolymph content, and especially, to identify its different hemocytes. Moreover, many papers have already shown molecules whose source is the hemolymph and their very interesting activities and biomedical applications, for example, antifungal and antibacterial activities. A 2D-SDS-PAGE of brown spider hemolymph showed approximately 111 spots for pH 3-10 and 150 spots for pH 4-7. A lectin-blotting assay showed that hemolymph carbohydrate residues were similar to those found in venom. Several types of TAG and DAG phospholipids were found in the hemolymph and characterized by HPTLC and mass spectrometry. Four different hemocytes were characterized in Loxosceles intermedia hemolymph: prohemocyte, plasmatocyte, granulocyte and adipohemocyte. This paper opens new possibilities on toxinology, studying an unknown biological material, and it characterizes a source of molecules with putative biotechnological applications.
这是对巴西游走蛛属蜘蛛血淋巴的首次研究。这类动物在全球范围内导致了大量的中毒事件。关于巴西游走蛛毒液已经发表了多项研究，不仅在生物学和生物化学特性方面，而且在结构、遗传和系统发育研究方面，对毒液及其毒素的了解都相当多。然而，关于巴西游走蛛血淋巴的文献却不存在。本研究的主要目的是对血淋巴成分进行生化表征，特别是鉴定其不同的血细胞。此外，许多论文已经展示了源自血淋巴的分子及其非常有趣的活性和生物医学应用，例如抗真菌和抗菌活性。棕色蜘蛛血淋巴的二维SDS - PAGE分析显示，pH值为3 - 10时约有111个斑点，pH值为4 - 7时约有150个斑点。凝集素印迹分析表明，血淋巴中的碳水化合物残基与毒液中的相似。在血淋巴中发现了几种类型的甘油三酯（TAG）和甘油二酯（DAG）磷脂，并通过高效薄层层析（HPTLC）和质谱进行了表征。在中间型巴西游走蛛的血淋巴中鉴定出了四种不同的血细胞：原血细胞、浆细胞、粒细胞和脂肪血细胞。本文为毒素学研究开辟了新的可能性，研究一种未知的生物材料，并对具有潜在生物技术应用的分子来源进行了表征。
