酒精对小鼠植入前胚胎中H3K9乙酰化的影响。
Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos.
作者信息
Ding Fang, Chen Li, Liu Yong, Wu Feng-Rui, Ding Biao, Li Wen-Yong, Wang Rong
机构信息
School of Life Science, Anhui University, Hefei 230601, China;School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China.
School of biological and food engineering, Fuyang Teachers College, Fuyang 236037, China;Key Laboratory of Embryo Development and Reproductive Regulation in Anhui, Fuyang 236037, China.
出版信息
Dongwuxue Yanjiu. 2015 Jan 18;36(1):54-8. doi: 10.13918/j.issn.2095-8137.2015.1.54.
It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels (except at the 2-cell stage). This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early pre-implantation mouse embryos.
众所周知，长期过量饮酒有害，尤其对孕妇而言。在本研究中，以昆明小白鼠作为动物模型，采用间接免疫荧光法分析酒精对植入前早期胚胎的毒性作用。在MII期卵母细胞中未检测到H3K9乙酰化免疫荧光。桑椹胚阶段，处理组的H3K9乙酰化水平高于对照组，而囊胚阶段结果相反。体内胚胎的其他阶段未显示明显差异。对于体外胚胎，两个实验组在所有阶段几乎没有差异，且两组的H3K9乙酰化水平均呈上升趋势（2细胞阶段除外）。本研究表明，雌性过量摄入酒精会显著影响植入前早期胚胎的H3K9乙酰化水平。这些数据是了解酒精对植入前早期小鼠胚胎毒性的表观遗传机制的第一步。
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