Methods for assessing the in vitro transforming activity of NF-κB transcription factor c-Rel and related proteins.

Among NF-κB transcription factors, c-Rel and c-Rel-derived proteins, including v-Rel, are the only ones that have shown consistent and frank transforming activity in cell culture. In particular, viral, chicken, mouse, and human Rel proteins can rapidly transform primary chicken spleen and bone marrow cells. Overexpression of a human Rel protein missing a C-terminal transactivation domain can also enhance the transformed state of the human B-lymphoma cell line BJAB. As described in this chapter, these in vitro assays can be used to quantitatively assess the transforming activity of Rel proteins.