骨桥蛋白通过PI3K-AKT依赖性信号传导诱导白色前脂肪细胞向棕色脂肪细胞分化。
Osteopontin-induced brown adipogenesis from white preadipocytes through a PI3K-AKT dependent signaling.
作者信息
Zhong Xiao-Juan, Shen Xiao-Dan, Wen Jian-Bing, Kong Ying, Chu Jia-Jia, Yan Guo-Qiang, Li Teng, Liu Dan, Wu Meng-Qing, Zeng Guo-Hua, He Ming, Huang Qi-Ren
机构信息
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, PR China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, PR China.
出版信息
Biochem Biophys Res Commun. 2015 Apr 10;459(3):553-9. doi: 10.1016/j.bbrc.2015.02.153. Epub 2015 Mar 6.
Recent studies have shown that OPN (osteopontin) plays critical roles in cell survival, differentiation, bio-mineralization, cancer and cardiovascular remodeling. However, its roles in the differentiation of brown adipocytes and the underlying mechanisms remain unclear. Therefore, the aim of this study was to investigate the roles of OPN in the brown adipogenesis and the underlying mechanisms. It was shown that the OPN successfully induced the differentiation of 3T3-L1 white preadipocytes into the PRDM16(+) (PRD1-BF1-RIZ1 homologous domain containing 16) and UCP-1(+) (uncoupling protein-1) brown adipocytes in a concentration and time-dependent manner. Also, activation of PI3K (phosphatidylinositol 3-kinase)-AKT pathway was required for the OPN-induced brown adipogenesis. The findings suggest OPN plays an important role in promoting the differentiation of the brown adipocytes and might provide a potential novel therapeutic approach for the treatment of obesity and related disorders.
最近的研究表明，骨桥蛋白（OPN）在细胞存活、分化、生物矿化、癌症和心血管重塑中发挥着关键作用。然而，其在棕色脂肪细胞分化中的作用及潜在机制仍不清楚。因此，本研究的目的是探讨OPN在棕色脂肪生成中的作用及其潜在机制。结果表明，OPN能以浓度和时间依赖性方式成功诱导3T3-L1白色前脂肪细胞分化为PRDM16（含PRD1-BF1-RIZ1同源结构域16）和UCP-1（解偶联蛋白-1）阳性的棕色脂肪细胞。此外，OPN诱导的棕色脂肪生成需要PI3K（磷脂酰肌醇3激酶）-AKT通路的激活。这些发现提示OPN在促进棕色脂肪细胞分化中起重要作用，可能为肥胖及相关疾病的治疗提供一种潜在的新治疗方法。
Zotero 插件