Zhang Xiu-Feng, Chen Lei, Yang Qian-Fan, Sun Xiao-Ran, Chen Hong-Bo, Yang Guang, Tang Ya-Lin
Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Nov;34(11):3045-50.
Using cyanine dyes supramolecular as molecular probes to mark FA has important significance in life sciences and pharmaceutical chemistry, which can detect FA as drug efficacy mechanism and the change in physiological activity. In the present paper, we investigated supramolecular assembly and chiral inducement of cyanine dyes template by FA with absorption and circular dichroism (CD) spectra. The result suggests that FA can induce cyanine dyes from J-aggregation to monomer along with different colors change and has strong affinity with cyanine dye monomer. The template of FA not only can translate the chirality of MTC H-aggregation to other two states, but also induce ETC J-aggregation to molecular rearrangement and form left-handed helix of J-aggregates. Besides, the association of PTC with FA, i. e. binding to FA gave rise to the J-aggregation CD signals. Meanwhile, it was inferred that the meso substituent of cyanine dyes play an important role in the interaction between FA and the J-aggregation: the smaller the meso substituent, the higher the affinity interacted with FA. Clearly, the binding abilities between cyanine dyes and FA follow the order of MTC>ETC>PTC. These results support that the cyanine dyes supramolecular aggregates can be used as a kind of excellent molecular probes for specific recognition of FA and achieve the effect of visual inspection.
使用花菁染料超分子作为分子探针标记叶酸在生命科学和药物化学中具有重要意义，其可将叶酸作为药物作用机制及生理活性变化进行检测。在本文中，我们通过吸收光谱和圆二色光谱（CD）研究了叶酸对花菁染料模板的超分子组装和手性诱导。结果表明，叶酸可诱导花菁染料从J-聚集体转变为单体，同时伴有颜色变化，且与花菁染料单体具有很强的亲和力。叶酸模板不仅能将MTC H-聚集体的手性传递到其他两种状态，还能诱导ETC J-聚集体发生分子重排并形成J-聚集体的左手螺旋。此外，PTC与叶酸的缔合，即与叶酸结合产生了J-聚集体的CD信号。同时，据推断花菁染料的中位取代基在叶酸与J-聚集体的相互作用中起重要作用：中位取代基越小，与叶酸相互作用的亲和力越高。显然，花菁染料与叶酸之间的结合能力顺序为MTC>ETC>PTC。这些结果支持花菁染料超分子聚集体可作为一种优异的分子探针用于特异性识别叶酸并实现可视化检测效果。
