Duggavathi Raj, Siddappa Dayananda, Schuermann Yasmin, Pansera Melissa, Menard Isabelle J, Praslickova Dana, Agellon Luis B
Department of Animal Sciences, McGill University, Quebec H9X 3V9, Canada.
J Reprod Dev. 2015;61(3):237-40. doi: 10.1262/jrd.2014-139. Epub 2015 Mar 5.
The fatty acid binding protein 6 (Fabp6) is commonly regarded as a bile acid binding protein found in the distal portion of the small intestine and has been shown to be important in maintaining bile acid homeostasis. Previous studies have also reported the presence of Fabp6 in human, rat and fish ovaries, but the significance of Fabp6 in this organ is largely unknown. Therefore, we surveyed murine ovaries for Fabp6 gene expression and evaluated its role in ovarian function using mice with whole body Fabp6 deficiency. Here we show that the Fabp6 gene is expressed in granulosa and luteal cells of the mouse ovary. Treatment with gonadotropins stimulated Fabp6 gene expression in large antral follicles. The ovulation rate in response to superovulatory treatment in Fabp6-deficient mice was markedly decreased compared to wildtype (C57BL/6) mice. The results of this study suggest that expression of Fabp6 gene in granulosa cells serves an important and previously unrecognized function in fertility.
脂肪酸结合蛋白6（Fabp6）通常被认为是一种在小肠远端发现的胆汁酸结合蛋白，并且已被证明在维持胆汁酸稳态中起重要作用。先前的研究也报道了Fabp6在人、大鼠和鱼的卵巢中存在，但Fabp6在该器官中的意义在很大程度上尚不清楚。因此，我们检测了小鼠卵巢中Fabp6基因的表达，并使用全身Fabp6缺陷的小鼠评估了其在卵巢功能中的作用。在这里，我们表明Fabp6基因在小鼠卵巢的颗粒细胞和黄体细胞中表达。用促性腺激素处理可刺激大的窦状卵泡中Fabp6基因的表达。与野生型（C57BL/6）小鼠相比，Fabp6缺陷小鼠对超排卵处理的排卵率明显降低。这项研究的结果表明，颗粒细胞中Fabp6基因的表达在生育力方面起着重要且以前未被认识到的作用。
