Han Xia, Liu Lili, Niu Jiamin, Yang Jun, Zhang Zengtang, Zhang Zhiqiang
Department of Cardiology, Yantai Yuhuangding Hospital, Qingdao University Medical College Shandong, China ; Department of Cardiology, Laiwu People's Hospital Laiwu, Shandong, China.
Special Department of Laiwu People's Hospital Laiwu, Shandong, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):922-7. eCollection 2015.
Our aim was to investigate the association between single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor (VEGF) and coronary heart disease (CHD) susceptibility in Chinese Han population.
144 CHD patients and 150 healthy individuals were enrolled in the study. Three SNPs (936C/T, -460T/C and -634G/C) of VEGF were chose and then were genotyped with Sequenom time-of-flight mass spectrometry (TOFMS). Odds ratio (OR) with 95% confidence interval (CI) were used to evaluate the association of genotypes and haplotypes and CHD susceptibility.
The frequencies of -460T/C CC genotype (13.6%) was found higher in the case group than that of control group (6.7%), which indicated that CC genotype was a risk factor for CHD (OR=2.50, 95% CI=1.10-5.68). Correspondently, the C allele appeared to increase the risk of CHD (OR=1.54, 95% CI=1.07-2.22). For -634G/C polymorphism, the risk of the CC genotype carrier for CHD increased 2.24 fold compared to the wild genotype. Moreover, -634G/CC allele was significantly associated with CHD susceptibility (OR=1.65, 95% CI=1.15-2.36). In addition, +936C/T CT genotype and C allele appeared to be a genetic-susceptibility factors for CHD (OR=2.43, 95% CI=1.44-4.10; OR=1.95, 95% CI=1.26-3.02). The haplotype analysis showed that T-C-T, C-C-C and C-G-C haplotypes all could increase the risk for CHD (OR: 2.43, 2.77 and 2.33).
we concluded VEGF polymorphisms were associated with CHD susceptibility. Moreover, the haplotypes of T-C-T, C-C-C and C-G-C all could increase the risk for CHD.
本研究旨在探讨中国汉族人群中血管内皮生长因子（VEGF）单核苷酸多态性（SNP）与冠心病（CHD）易感性之间的关联。
本研究纳入了144例冠心病患者和150例健康个体。选取VEGF的3个SNP（936C/T、-460T/C和-634G/C），然后采用Sequenom飞行时间质谱（TOFMS）进行基因分型。采用比值比（OR）及95%置信区间（CI）评估基因型和单倍型与冠心病易感性的关联。
病例组中-460T/C CC基因型频率（13.6%）高于对照组（6.7%），提示CC基因型是冠心病的危险因素（OR=2.50，95%CI=1.10-5.68）。相应地，C等位基因似乎增加了冠心病的发病风险（OR=1.54，95%CI=1.07-2.22）。对于-634G/C多态性，CC基因型携带者患冠心病的风险比野生基因型增加了2.24倍。此外，-634G/C等位基因与冠心病易感性显著相关（OR=1.65，95%CI=1.15-2.36）。此外，+936C/T CT基因型和C等位基因似乎是冠心病的遗传易感性因素（OR=2.43，95%CI=1.44-4.10；OR=1.95，95%CI=1.26-3.02）。单倍型分析显示，T-C-T、C-C-C和C-G-C单倍型均能增加冠心病的发病风险（OR分别为2.43、2.77和2.33）。
我们得出结论，VEGF基因多态性与冠心病易感性相关。此外，T-C-T、C-C-C和C-G-C单倍型均能增加冠心病的发病风险。
