Chen Yuan, Han Yong, Wang Jun, Hua Ming-Ming, Gu Chao, Li Guo-Sheng, Zhang Xiang, Chen De-Hua
Ying Yong Sheng Tai Xue Bao. 2014 Sep;25(9):2623-8.
Taking Bt cotton Sikang 1 (a conventional cultivar), Sikang 3 (a hybrid cultivar) from China and 99B (a conventional cultivar), Daiza 1 (a hybrid cultivar) from USA as test materials, the effects of different high temperatures on thesquare Bt proteins expression and nitrogen metabolic physiology were investigated. The results showed that the square Bt protein contents of the four cultivars decreased significantly above 38 °C compared with that at 32 °C. The higher the temperature was above 38 °C, the greater the reduction extent of the Bt protein content was. The square Bt protein contents of the hybrid cultivars were higher than that of the conventional cultivars, and were less reduced under the high temperature stress. The cultivars with bigger reductions in Bt protein content also showed greater reductions in the square soluble protein contents, pyruvic transaminase activities and glutamic oxaloacetic transaminase activities, while larger increments were detected for the square free amino acid contents, proteinsase activities and peptidase activities.
以中国的转Bt基因棉花泗抗1号（常规品种）、泗抗3号（杂交品种）以及美国的99B（常规品种）、岱杂1号（杂交品种）为试验材料，研究了不同高温对棉铃Bt蛋白表达及氮代谢生理的影响。结果表明，与32℃相比，4个品种在38℃以上时棉铃Bt蛋白含量显著降低。38℃以上温度越高，Bt蛋白含量降低幅度越大。杂交品种的棉铃Bt蛋白含量高于常规品种，且在高温胁迫下降低幅度较小。Bt蛋白含量降低幅度较大的品种，其棉铃可溶性蛋白含量、谷丙转氨酶活性和谷草转氨酶活性降低幅度也较大，而棉铃游离氨基酸含量、蛋白酶活性和肽酶活性增幅较大。
