Li Bin, Wang Li, Ibrahim Muhammad, Ge Mengyu, Wang Yanli, Mannan Shazia, Asif Muhammad, Sun Guochang
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China,
Arch Microbiol. 2015 Jun;197(5):673-82. doi: 10.1007/s00203-015-1100-9. Epub 2015 Mar 13.
Membrane proteins (MPs) of plant pathogenic bacteria have been reported to be able to regulate many essential cellular processes associated with plant disease. The aim of the current study was to examine and compare the expression of MPs of the rice bacterial pathogen Acidovorax avenae subsp. avenae strain RS-1 under Luria-Bertani (LB) medium, M9 medium, in vivo rice plant conditions and leaf extract (LE) medium mimicking in vivo plant condition. Proteomic analysis identified 95, 72, 75, and 87 MPs under LB, in vivo, M9 and LE conditions, respectively. Among them, six proteins were shared under all tested growth conditions designated as abundant class of proteins. Twenty-six and 21 proteins were expressed uniquely under in vivo versus LB medium and LE versus M9 medium, respectively, with 17 proteins common among these uniquely induced proteins. Moreover, most of the shared proteins are mainly related to energy metabolism, transport of small molecules, protein synthesis and secretion as well as virulence such as NADH, OmpA, secretion proteins. Therefore, the result of this study not only suggests that it may be an alternate method to analyze the in vivo expression of proteins by using LE medium to mimic plant conditions, but also reveals that the two sets of differentially expressed MPs, in particular the common MPs between them, might be important in energy metabolism, stress response and virulence of A. avenae subsp. avenae strain RS-1.
据报道，植物病原细菌的膜蛋白（MPs）能够调节许多与植物病害相关的重要细胞过程。本研究的目的是检测和比较水稻细菌性病原菌燕麦嗜酸菌燕麦亚种RS-1在Luria-Bertani（LB）培养基、M9培养基、水稻植株体内条件以及模拟植株体内条件的叶提取物（LE）培养基下MPs的表达情况。蛋白质组学分析分别在LB、体内、M9和LE条件下鉴定出95、72、75和87种MPs。其中，有6种蛋白质在所有测试生长条件下均有表达，被指定为丰富类蛋白质。分别有26种和21种蛋白质在体内与LB培养基以及LE与M9培养基下独特表达，在这些独特诱导的蛋白质中有17种是共同的。此外，大多数共享蛋白质主要与能量代谢、小分子运输、蛋白质合成与分泌以及毒力相关，如NADH、OmpA、分泌蛋白。因此，本研究结果不仅表明使用LE培养基模拟植物条件可能是分析蛋白质体内表达的一种替代方法，还揭示了两组差异表达的MPs，特别是它们之间的共同MPs，可能在燕麦嗜酸菌燕麦亚种RS-1的能量代谢、应激反应和毒力方面具有重要作用。
