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小脑顶核刺激通过诱导一种新型微小RNA(rno-miR-676-1)对大脑中动脉闭塞大鼠发挥神经保护作用。

Fastigial nucleus stimulation regulates neuroprotection via induction of a novel microRNA, rno-miR-676-1, in middle cerebral artery occlusion rats.

作者信息

Pang Xiao-Min, Liu Jing-Li, Li Jin-Pin, Huang Li-Gang, Zhang Lei, Xiang Hui-Yao, Feng Ling-Bo, Chen Chun-Yong, Li Sheng-Hua, Su Sheng-You

机构信息

Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, China.

Department of Neurology, Dongguan Kanghua Hospital, Dongguan, Guangdong, China.

出版信息

J Neurochem. 2015 Jun;133(6):926-34. doi: 10.1111/jnc.13094. Epub 2015 Apr 10.

Abstract

Previous studies have shown that fastigial nucleus stimulation (FNS) reduces tissue damage resulting from focal cerebral ischemia. Although the mechanisms of neuroprotection induced by FNS are not entirely understood, important data have been presented in the past two decades. MicroRNAs (miRNAs) are a newly discovered group of non-coding small RNA molecules that negatively regulate target gene expression and are involved in the regulation of cell proliferation and cell apoptosis. To date, no studies have demonstrated whether miRNAs can serve as mediators of the brain's response to FNS, which leads to endogenous neuroprotection. Therefore, this study investigated the profiles of FNS-mediated miRNAs. Using a combination of deep sequencing and microarray with computational analysis, we identified a novel miRNA in the rat ischemic cortex after 1 h of FNS. This novel miRNA (PC-3p-3469_406), herein referred to as rno-miR-676-1, was upregulated in rats with cerebral ischemia after FNS. In vivo observations indicate that this novel miRNA may have antiapoptotic effects and contribute to neuroprotection induced by FNS. Our study provides a better understanding of neuroprotection induced by FNS. MicroRNA (miRNA) is defined as a small non-coding RNA that fulfills both the expression and biogenesis criteria. Here, we describe a novel miRNA in the rat ischemic cortex expressed after 1 h of fastigial nucleus stimulation (FNS). The miRNA was functionally characterized by secondary structure, quantitative expression, the conservation analysis, target gene analysis, and biological functions. We consider rno-miR-676-1 to be a true microRNA and present evidence for its neuroprotective effects exerted after induction by FNS.

摘要

先前的研究表明,小脑顶核刺激(FNS)可减少局灶性脑缺血导致的组织损伤。尽管FNS诱导神经保护的机制尚未完全明确,但在过去二十年中已有重要数据被提出。微小RNA(miRNA)是新发现的一类非编码小RNA分子,可负向调节靶基因表达,并参与细胞增殖和细胞凋亡的调控。迄今为止,尚无研究表明miRNA是否可作为大脑对FNS反应的介质,从而导致内源性神经保护。因此,本研究调查了FNS介导的miRNA谱。通过深度测序、微阵列结合计算分析,我们在FNS 1小时后的大鼠缺血皮层中鉴定出一种新型miRNA。这种新型miRNA(PC-3p-3469_406),在此称为rno-miR-676-1,在FNS后的脑缺血大鼠中上调。体内观察表明,这种新型miRNA可能具有抗凋亡作用,并有助于FNS诱导的神经保护。我们的研究有助于更好地理解FNS诱导的神经保护作用。微小RNA(miRNA)被定义为满足表达和生物合成标准的小非编码RNA。在此,我们描述了在小脑顶核刺激(FNS)1小时后大鼠缺血皮层中表达的一种新型miRNA。通过二级结构、定量表达、保守性分析、靶基因分析和生物学功能对该miRNA进行了功能表征。我们认为rno-miR-676-1是一种真正的微小RNA,并提供了其在FNS诱导后发挥神经保护作用的证据。

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