Walaas L, Kindblom L G
Hum Pathol. 1985 Jan;16(1):6-18. doi: 10.1016/s0046-8177(85)80208-2.
A correlative cytologic and histologic study of 12 benign lipomatous tumors and 15 liposarcomas (well-differentiated, myxoid, round cell, and pleomorphic) is presented. In two cases the fine needle aspiration material was embedded in Epon for light and electron microscopic examination. Good correlation was found between the histologic and cytologic findings in the fine needle aspiration material. Pitfalls in the cytologic diagnosis of regressively changed lipoma, intramuscular lipoma, angiolipoma, hibernoma, and lipoblastoma, which may lead to an erroneous diagnosis of liposarcoma, are illustrated. The cytologic appearances of the liposarcomas varied with histologic type, although in all of these tumors the main criterion was the presence of atypical multivacuolated lipoblasts with characteristically scalloped nuclei. Staining of the aspirated material with Alcian blue at varying pH levels for characterization of the glycosaminoglycan content may help in the distinction of myxoid liposarcomas from myxoid chondromatous tumors and chordomas. May-Grünewald-Giemsa staining is considered the most useful staining method, while fat staining is considered of limited or no value in the cytologic diagnosis of lipomatous tumors. Epon embedding of fine needle aspirates for light and electron microscopic examination seems to be a useful diagnostic technique.
本文介绍了对12例良性脂肪瘤和15例脂肪肉瘤（高分化型、黏液型、圆细胞型和多形性）进行的细胞学与组织学相关性研究。在两例病例中，细针穿刺抽吸物被包埋于环氧树脂中，用于光镜和电镜检查。在细针穿刺抽吸物的组织学和细胞学检查结果之间发现了良好的相关性。文中阐述了退行性变脂肪瘤、肌内脂肪瘤、血管脂肪瘤、冬眠瘤和成脂细胞瘤在细胞诊断中的陷阱，这些可能导致脂肪肉瘤的误诊。脂肪肉瘤的细胞形态随组织学类型而异，尽管在所有这些肿瘤中，主要标准是存在具有特征性扇贝样核的非典型多泡性脂肪母细胞。用不同pH值的阿尔辛蓝对抽吸物进行染色以表征糖胺聚糖含量，可能有助于区分黏液型脂肪肉瘤与黏液型软骨瘤性肿瘤和脊索瘤。May-Grünewald-Giemsa染色被认为是最有用的染色方法，而脂肪染色在脂肪瘤性肿瘤的细胞诊断中被认为价值有限或无价值。将细针穿刺抽吸物包埋于环氧树脂中进行光镜和电镜检查似乎是一种有用的诊断技术。
