眼轮匝肌组织学的区域差异。

Regional Variations in Orbicularis Oculi Histology.

作者信息

Costin Bryan R, Plesec Thomas P, Kopplin Laura J, Chundury Rao V, McBride Jennifer M, Levine Mark R, Perry Julian D

机构信息

*Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio and †Department of Anatomic Pathology, Cleveland Clinic, Cleveland, Ohio; ‡Casey Eye Institute, Oregon Health and Science University, Portland, Oregon; §Department of Anatomy, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio; ‖Lorain Institute, Cleveland Clinic, Lorain, Ohio.

出版信息

Ophthalmic Plast Reconstr Surg. 2015 Jul-Aug;31(4):325-7. doi: 10.1097/IOP.0000000000000430.

DOI:10.1097/IOP.0000000000000430

PMID:25794026

Abstract

PURPOSE

To investigate and compare the histologic compositions of the pretarsal, preseptal, and orbital orbicularis oculi muscle (OOM) using nonpreserved, fresh-frozen, human cadavers.

METHODS

The OOM was exposed using sharp and blunt dissection. A metric ruler was used to measure and mark 0.5 cm × 1 cm samples from each portion of the right, superior OOM. Samples were excised, fixed in formalin, and completely embedded in paraffin. Five-micrometer-thick, hematoxylin- and eosin-stained sections were generated for each sample and analyzed by an anatomical pathologist. The relative percentages of the 4 main tissue types (skeletal muscle, fibrous tissue, adipose tissue, and neurovascular tissue) were quantified.

RESULTS

Forty-two samples were obtained from 14 Caucasian cadavers. On average, the pretarsal samples were composed of 83.5% skeletal muscle, 0.0% adipose, 5.0% neurovascular, and 11.5% fibrous tissue. Average preseptal OOM was 46.5% skeletal muscle, 12.7% adipose, 9.2% neurovascular, and 31.5% fibrous tissue. The orbital OOM was, on average, 42.7% skeletal muscle, 32.7% adipose tissue, 6.9% neurovascular, and 17.7% fibrous tissue.

CONCLUSIONS

The OOM represents a histologically heterogeneous structure.

摘要

目的

使用未保存的新鲜冷冻人体尸体，研究并比较睑前、眶隔前及眶部眼轮匝肌（OOM）的组织学组成。

方法

采用锐性和钝性解剖暴露OOM。使用公制尺从右侧眼轮匝肌上部的各部分测量并标记出0.5厘米×1厘米的样本。切除样本，用福尔马林固定，并完全包埋于石蜡中。为每个样本制作5微米厚的苏木精-伊红染色切片，并由一名解剖病理学家进行分析。对4种主要组织类型（骨骼肌、纤维组织、脂肪组织和神经血管组织）的相对百分比进行量化。

结果

从14具白种人尸体上获取了42个样本。平均而言，睑前样本由83.5%的骨骼肌、0.0%的脂肪、5.0%的神经血管和11.5%的纤维组织组成。眶隔前眼轮匝肌平均为46.5%的骨骼肌、12.7%的脂肪、9.2%的神经血管和31.5%的纤维组织。眶部眼轮匝肌平均为42.7%的骨骼肌、32.7%的脂肪组织、6.9%的神经血管和17.7%的纤维组织。