Wu Feng-Zhi, Li Min, Cao Peng, Ma Ya-Fei, Wang Li-Li
Ying Yong Sheng Tai Xue Bao. 2014 Oct;25(10):2861-7.
With wheat as the donor plant and cucumber as the receptor plant, this study investigated the effects of root exudates from wheat cultivars with different allelopathic potentials (positive or negative) and companion cropping with wheat on soil fungal community structure by PCR-DGGE method and cucumber growth. Results showed that the wheat root exudates with positive allelopathic potential increased height and stem diameter of cucumber seedlings significantly, compared to the control seedlings (W) after 6 days and 12 days treatment, respectively. Also, wheat root exudates with both positive and negative allelopathic potential increased the seedling height of cucumber significantly after 18 days treatment. The wheat root exudates with different allelopathic potentials decreased the band number, Shannon and evenness indices of soil fungal community significantly in cucumber seedling rhizosphere, and those in the soil with the control seedlings (W) were also significantly higher than that in the control soil without seedlings (Wn) after 6 days treatment. The band number, Shannon and evenness indices in all the treatments were significantly higher than those in the control soil without seedlings (Wn) after 18 days treatment. Companion cropping with negative allelopathic potential wheat decreased the Shannon and evenness indices of soil fungi community significantly in the cucumber seedling rhizosphere, suggesting the wheat root exudates and companion cropping with wheat changed soil fungal community structure in the cucumber seedling rhizosphere. The results of DGGE map and the principal component analysis showed that companion cropping with wheat cultivars with different allelopathic potentials changed soil fungal community structure in cucumber seedling rhizosphere.
以小麦为供体植物、黄瓜为受体植物，本研究采用PCR-DGGE方法，研究了具有不同化感潜力（正或负）的小麦品种根系分泌物以及与小麦间作，对土壤真菌群落结构和黄瓜生长的影响。结果表明，具有正化感潜力的小麦根系分泌物，分别在处理6天和12天后，与对照幼苗（W）相比，显著增加了黄瓜幼苗的株高和茎粗。此外，具有正、负化感潜力的小麦根系分泌物在处理18天后，均显著增加了黄瓜的幼苗株高。具有不同化感潜力的小麦根系分泌物，显著降低了黄瓜幼苗根际土壤真菌群落的条带数、香农指数和均匀度指数，且在处理6天后，对照幼苗（W）土壤中的这些指标也显著高于无幼苗的对照土壤（Wn）。处理18天后，所有处理的条带数、香农指数和均匀度指数均显著高于无幼苗的对照土壤（Wn）。与具有负化感潜力的小麦间作，显著降低了黄瓜幼苗根际土壤真菌群落的香农指数和均匀度指数，表明小麦根系分泌物和与小麦间作改变了黄瓜幼苗根际土壤真菌群落结构。DGGE图谱和主成分分析结果表明，与具有不同化感潜力的小麦品种间作，改变了黄瓜幼苗根际土壤真菌群落结构。
