Knirschova Renata, Novakova Renata, Mingyar Erik, Bekeova Carmen, Homerova Dagmar, Kormanec Jan
Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
J Microbiol Methods. 2015 Jun;113:1-3. doi: 10.1016/j.mimet.2015.03.017. Epub 2015 Mar 20.
The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.
整合型启动子探针质粒pBPSA1是利用无启动子的金色链霉菌CCM3239 bpsA基因构建的，该基因编码一种用于生物合成蓝色色素靛蓝素的非核糖体肽合成酶。bpsA还用于制备pAMR4质粒，以便在链霉菌中缺失基因，并能轻松鉴定双交换重组。
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